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Recombinant, mouse γ-interferon (γ-IFN) expressed in E. coli as a single, non-glycosylated polypeptide containing 134 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
Catalogue Number
407320
Brand Family
Calbiochem®
Synonyms
Interferon γ precursor, IFN-γ, rmIFN-γ
References
References
Alvarado, J., et al. 2005. Immunol. Lett.99, 109. Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med.43, 499. Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal.19, 128.
Reported as IU/mg on a lot-specific basis using a cytopathic effect inhibition assay.
Purity
≥95% by RP-HPLC, FPLC, and SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Protect from Moisture
Protect from moisture
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for 2-7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
Alvarado, J., et al. 2005. Immunol. Lett.99, 109. Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med.43, 499. Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal.19, 128.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
05-March-2009 JSW
Synonyms
Interferon γ precursor, IFN-γ, rmIFN-γ
Description
Recombinant, mouse γ-interferon (γ-IFN) expressed in E. coli as a single, non-glycosylated polypeptide containing 134 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
Form
Lyophilized
Formulation
Lyophilized from 0.5X PBS, pH 7.2.
Purity
≥95% by RP-HPLC, FPLC, and SDS-PAGE
Biological activity
Reported as IU/mg on a lot-specific basis using a cytopathic effect inhibition assay.
Solubility
Reconstitute with sterile water to ≥100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.
Storage
Protect from moisture
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for 2-7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Alvarado, J., et al. 2005. Immunol. Lett.99, 109. Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med.43, 499. Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal.19, 128.
