Clarification made easy with pDADMAC flocculation reagent

In today's biopharmaceutical industry, high-density cell culture processes pose a significant threat to clarification and purification operations. With high solid content and an increased number of submicron particles, the separation technique to remove all solids is complex.

The use of flocculation in harvest is one way to overcome this challenge, with GMP-purified cationic polymer poly (diallyldimethylammonium chloride) [pDADMAC] being an effective flocculation reagent. Upon addition to the cell culture feed stream in a concentration between 0.01 and 0.05%, it rapidly flocculates the negatively charged cells and cellular debris into larger particles via an ionic interaction mechanism.

Acid treated and polymer-flocculent treated feeds shift the particle size distribution towards larger particle sizes by agglomerating smaller particulates into larger particles. Traditional depth filters are not suited to the particle size distributions o such pre-treated feeds. Our Clarisolve® depth filters have been optimized to match the particle size distribution of typical pre-treated feeds, resulting in improved filtration performance not seen with traditional clarification depth filters. Together with our pDADMAC reagent, they provide an effective solution for harvesting high density cell cultures.

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