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69662 pET-16b DNA - Novagen

Novagen's pET-16b vector carries an N-terminal His•Tag® sequence followed by a Factor Xa site and three cloning sites.

pET-16b DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69662
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69662-3CN
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      		 Plastic ampoule 10 μg
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      Description
      OverviewThe pET-16b vector carries an N-terminal His•Tag® sequence followed by a Factor Xa site and three cloning sites. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB046).

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69662
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      69662-3CN 04055977274509

      Documentation

      pET-16b DNA - Novagen Certificates of Analysis

      TitleLot Number
      69662

      Citations

      Title
    • Rossi, F., et al. 2008. Journal of Biological Chemistry 283, 3559.
    • Kröning, N., et al. 2007. Journal of Biological Chemistry 282, 14018.
    • Otte, M.M., et al. 2007. Journal of Bacteriology 189, 7310.
    • Plamondon, P., et al. 2007. Infection and Immunity 75, 2929.
    • Sinha, K.M., et al. 2007. Journal of Biological Chemistry 282, 15114.
    • Yu, Z., et al. 2007. Nucleic Acids Research 35, 2107.
    • Colasante, C., et al. 2006Eukaryotic Cell 5, 1194.
    • Roelofs, M.F., et al. 2006. Journal of Immunology 176, 7021.
    • Ashida, H., et al. 2005. Plant and Cell Physiology 46, 557.
    • Beernink, P.T, et al. 2005. Journal of Biological Chemistry 280, 30206.
    • Bruck, I., et al. 2005. Journal of Biological Chemistry 280, 18152.
    • Funa, N., et al. 2005. Journal of Biological Chemistry 280, 14514.
    • Hambsch, B., et al. 2005. Journal of Biological Chemistry 280, 15888.
    • Iwata, T., et al. 2005. Clinical Cancer Research 11, 3949.
    • Korepanova, A., et al. 2005. Protein Science 14, 148.
    • Liu, D., et al. 2005. Cancer Research 65, 3796.
    • O'Brien, P.J., et al. 2005. Clinical Chemistry 51, 351.
    • Annabelle Varrot, A., et al. 2005. Journal of Biological Chemistry 280, 20181.
    • Wilkinson, A., et al. 2005. Biochimica et Biophysica Acta 1749, 113.
    • Zhao, X., et al. 2005. Cancer Research 65, 2125.
    • Zhu H., and Shuman S. 2005. Journal of Biological Chemistry 280, 418.
    • Andrews, S.R., et al. 2004. Journal of Biological Chemistry 279, 54369.
    • Kobayashi, D., et al. 2003.Plant and Cell Physiology 44, 269.
    • Huhtinen, K., et al. 2002. Journal of Biological Chemistry 277, 3424.
    • Funa, N., et al. 1999. Nature 400, 897.
    • Zheng N. and Gierasch L.M. 1997. Molecular Cell 1, 79.
    • Deveraux, Q., et al. 1995. Journal of Biological Chemistry 270, 23726.
    • Ong, O.C., et al. 1995. Journal of Biological Chemistry 270, 8495.
    • Gotoh, Y., et al. 1994. Oncogene 9, 1891.
    • Franke, C.A. and Hruby, D.E.1993. Protein Expression and Purification 4, 101.
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      User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Vector Map

      Title
      TB046VM pET-16b Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors