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23-044 VHL Protein Complex, Active, 10 µg

Active complex of five (5) recombinant human enzymes: VHL amino acids 54-end, full length Elongin C, full length Elongin B, full length Cul2, & full length Rbx1. For use in Enzyme Assays. Function

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VHL Protein Complex, Active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
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23-044
10 µg  
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      Overview

      Key Spec Table

      Key ApplicationsSpeciesCascade Component
      Enzyme AssaysHuman E3
      Description
      Catalogue Number23-044
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionVHL Protein Complex, Active, 10 µg
      Alternate Names
      • VCB-Cul2 complex
      • ECV complex
      • CBCVHL
      Background InformationThe VHL complex is a multi-subunit ubiquitin ligase composed of VHL, Elongin B, Elongin C, Cul2 and Rbx1. The VHL protein serves as the substrate recognition component and is linked by Elongin C to a heterodimeric Cul2/Rbx1 module that functions as a potent activator of the ubiquitination of target proteins by an E2 conjugating enzyme. Elongin B interacts with the complex through Elongin C and appears to stabilize the binding of Elongin C to VHL. The primary function of the VHL complex is to regulate HIF (hypoxia inducible factor) activity by targeting the hydroxylated HIF-1α subunit for ubiquitination and rapid proteasomal degradation under normoxic conditions. It therefore plays an important role in the regulation of hypoxia-inducible genes such as the vascular endothelial growth factor (VEGF) and glucose transport-1 (Glut-1). Mutations in VHL are associated with the inherited von Hippel-Lindau (VHL) cancer syndrome and numerous forms of renal cell carcinoma.
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive complex of five (5) recombinant human enzymes: VHL amino acids 54-end, full length Elongin C, full length Elongin B, full length Cul2, & full length Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
      Key Applications
      • Enzyme Assays
      Biological Information
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Cascade ComponentE3
      Entrez Gene Number
      Purification MethodPurified using glutathione sepharose.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Packaging Information
      Material Size10 µg
      Global Trade Item Number
      Catalogue Number GTIN
      23-044 04053252294884