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23-025M UbcH4 Protein, active, 250 µg

Active, full length, recombinant human UbcH4. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

Key Applications: Enzyme Assays
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23-025M
250 µg  
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      Overview

      Key Spec Table

      Key ApplicationsSpeciesCascade Component
      Enzyme AssaysHuman E2
      Description
      Catalogue Number23-025M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionUbcH4 Protein, active, 250 µg
      OverviewAXIS Axon Isolation Devices, 150 µm long channels, 5 devices
      Alternate Names
      • Ubiquitin-conjugating enzyme E2 D2
      • Ubiquitin-protein ligase D2
      • UbcH5b
      • Ubiquitin-conjugating enzyme E2-17kDa 2
      • UBE2D2
      Background InformationUbiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate. The UbcH5 family (UbcH5a, UbcH5b/UbcH4 and UbcH5c) have been shown to be the most active class of E2 enzymes in cell extracts and are associated with the regulation of a number of proteins associated with cell signalling, including p53, IκBα and β-catenin.
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActive, full length, recombinant human UbcH4. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
      Key Applications
      • Enzyme Assays
      Biological Information
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Cascade ComponentE2
      Entrez Gene Number
      Purification MethodPurified using immobilized metal affinity chromatography.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Packaging Information
      Material Size250 µg
      Global Trade Item Number
      Catalogue Number GTIN
      23-025M 04053252294419