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QIA99 Soluble Tissue Plasminogen Activator ELISA Kit

QIA99
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Overview

Key Spec Table

Species ReactivityDetection Methods
HColorimetric

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QIA99-1KITCN
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      Description
      OverviewUseful for the quantitative detection of soluble tissue-type plasminogen activator (t-PA). Altered levels of t-PA have been implicated in the progression of Grave’s disease, liver disorders, diabetic retinopathy, cardiovascular disease, thrombosis, myocardial infarction, and stroke.
      Catalogue NumberQIA99
      Brand Family Calbiochem®
      Application Data
      Materials Required but Not Delivered 5 and 10 ml graduated pipettes
      10 to 1,000 µl adjustable single channel micropipettes with disposable tips
      50 to 300 µl adjustable multichannel micropipette with disposable tips
      Multichannel micropipette reservoir
      Beakers, flasks, and cylinders necessary for preparation of reagents
      Multichannel wash bottle or automatic wash system for delivery of wash solution
      Microwell strip reader capable of reading at 450 nm with 620 nm as optional reference wavelength
      Glass-distilled or deionized water
      Calculator with program to perform linear regression analysis
      References
      ReferencesLi, Y., et al. 1998. Eur. J. Clin. Invest. 28, 1050.
      Skrha, J., et al. 1994. Clin. Chim. Acta. 229, 5.
      Okabe, K., et al. 1992. Gastroenterol. Jpn. 27, 61.
      Dano, K., et al. 1988. In Tissue-Type Plasminogen Activator (t-PA): Physiological and Clinical Aspects (Kluft, C.,
      ed.) pp. 20-46, CRC Press, Boca Raton, Florida.
      Kluft, C. 1988. In Tissue-Type Plasminogen Activator (t-PA): Physiological and Clincal Aspects(Kluft, C., ed.) pp. 47-49, CRC Press, Boca Raton, Florida.
      Kruithof, E.K.O. 1988. In Tissue-Type Plasminogen Activator (t-PA): Physiological and Clincal Aspects (Kluft, C.,
      ed.) pp. 190-210, CRC Press, Boca Raton, Florida.
      Mehta, J., et al. 1987. J. Am. Coll. Cardiol. 9, 263.
      Wilman, B., et al. 1986. J. Lab. Clin. Med. 105, 265.
      Product Information
      Detection methodColorimetric
      Form96 Tests
      Format96-well plate
      Kit containsPre-Coated Microtiter Plate Strips, Anti-t-PAI/HRP-Conjugate, t-PA Standard, Wash Buffer Concentrate, Assay Buffer Concentrate, Sample Diluent, Substrate Solutions I and II, Stop Solution, Plate Covers, and a user protocol
      Quality LevelMQ100
      Biological Information
      Assay range16 - 1000 pg/ml
      Assay time2.5 h
      Sample TypeCell culture supernatant, serum, plasma, or other biological fluids
      Species Reactivity
      • Human
      Physicochemical Information
      Sensitivity8 pg/ml
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36-45

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Storage ConditionsUpon arrival store the entire contents of the kit at 4°C.
      Do not freeze Yes
      Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
      Supplemental Information
      Kit containsPre-Coated Microtiter Plate Strips, Anti-t-PAI/HRP-Conjugate, t-PA Standard, Wash Buffer Concentrate, Assay Buffer Concentrate, Sample Diluent, Substrate Solutions I and II, Stop Solution, Plate Covers, and a user protocol
      Global Trade Item Number
      Catalogue Number GTIN
      QIA99-1KITCN 04055977209266