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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

71049 MilliporeProteinase K Solution, 600 mAU/ml

Efficient removal of proteins from nucleic acid solutions

Proteinase K Solution, 600 mAU/ml MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

CoA
Brochures
User Protocol
Citations

Recommended Products

Overview

Replacement Information

Pricing & Availability

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Description
Overview	Proteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/ml for protein removal and enzyme inactivation, and up to 2 mg/ml for tissue treatment. The Proteinase K, Lyophilized powder can be prepared as a 20 mg/ml stock solution in water and stored in aliquots at -20°C. The enzyme is also available as a ready-to-use concentrated stock solution (600 mAU/ml) that is convenient for routine use in most applications. 1 mg of Proteinase K is the equivalent of 30 mAU (AU = Anson unit). The Novagen Proteinase K products are free of detectable DNase and RNase
Catalogue Number	71049
Brand Family	Novagen®

References

Product Information
Unit of Definition	One AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C.
Quality Level	MQ400

Applications

Biological Information

Physicochemical Information
Contaminants	Free of detectable DNase and RNase.

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Shipped with Blue Ice or with Dry Ice
Toxicity	Harmful
Storage	-20°C
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
71049-4CN	07790788053000

Documentation

Proteinase K Solution, 600 mAU/ml Certificates of Analysis

Title	Lot Number
71049	Enter Lot Number

Brochure

Title
High fidelity gene amplification
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

Citations

Title

Shannon M. Anderson, et al. (2007) New markers for murine memory B cells that define mutated and unmutated subsets. Journal of Experimental Medicine 204, 2103-2114.

Andrew A. Horwitz, et al. (2007) ATP-induced structural transitions in PAN, the proteasome-regulatory ATPase complex in Archaea. Journal of Biological Chemistry 282, 22921-22929.

User Protocols

Title
TB271 Proteinase K

Related Products

Catalogue Number

Description

70663

Proteinase K, Lyophilized

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