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69149 Perfect Protein™ Markers, 15-150 kDa

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69149
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69149-3CN
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      Description
      OverviewThe Perfect Protein™ Markers are a novel set of recombinant proteins with defined sizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gel electrophoresis, the Perfect Protein Markers enable highly accurate size determination of unknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).

      The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75, 100, and 150 kDa. Each vial contains 400 µg protein. A 5-µl load contains 0.5 µg for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 µg).

      The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 µg protein. A 5-µl load contains 0.5 µg for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 µg).


      The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
      Catalogue Number69149
      Brand Family Novagen®
      References
      Product Information
      FormLiquid
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Toxic
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      69149-3CN 07790788052522

      Documentation

      Perfect Protein™ Markers, 15-150 kDa Certificates of Analysis

      TitleLot Number
      69149

      Citations

      Title
    • Kenji Kojima, et al. (2008) Roles of functional and structural domains of hepatocyte growth factor activator inhibitor type 1 in the inhibition of matriptase. Journal of Biological Chemistry 283, 2478-2487.
    • Athmane Teghanemt, et al. (2008) Novel roles in human MD-2 of phenylalanines 121 and 126 and tyrosine 131 in activation of toll-like receptor 4 by endotoxin. Journal of Biological Chemistry 283, 1257-1266.
    • Jim F. White, et al. (2007) Dimerization of the class A G protein-coupled neurotensin receptor NTS1 alters G protein interaction. Procedings of the National Academy of Science 104, 12199-12204.
    • Dequan Chen, et al. (2005) Surface calreticulin mediates muramyl dipeptide-induced apoptosis in RK13 cells. Journal of Biological Chemistry 280, 22425-22436.
    • Larisa Tsavaler, et al. (2001) Trp-p8, a novel prostate-specific gene, Is up-regulated in prostate cancer and other malignancies and shares high homology with transient receptor potential calcium channel proteins. Cancer Research 61, 3760-3769.
    • Kelvin Nurse, et al. (1995) Purification, cloning, and properties of the tRNA ψ55 synthase from Escherichia coli. RNA 1,.
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      Categories

      Life Science Research > Protein Detection and Quantification > Western Blotting > Western Blotting Kits and Reagents