71346 Sigma-AldrichOrigami™ 2(DE3)pLysS Competent Cells - Novagen
Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.
More>> Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression. Less<<MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview
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Pricing & Availability
| Catalogue Number | Availability | Packaging | Qty/Pack | Price | Quantity | |
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| 71346-3CN |
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Plastic ampoule | 0.4 ml |
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| 71346-4CN |
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Glass bottle | 1 ml |
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| Description | |
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| Overview | Origami™ 2 host strains are K-12
derivatives that have mutations in both the
thioredoxin reductase (trxB) and glutathione
reductase (gor) genes, which greatly enhance
disulfide bond formation in the E. coli
cytoplasm.
The Origami
2 strains are kanamycin sensitive; like the
original Origami strains, the gor mutation is still selected
for by tetracycline. To reduce the possibility of
disulfide bond formation between molecules,
strains containing mutations in trxB and gor are
recommended only for the expression of proteins
that require disulfide bond formation for proper
folding.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. |
| Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139
ahpC galE galK rpsL F′[lac+ lacIq pro] (DE3) gor522::Tn10
trxB pLysS (CamR, StrR, TetR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com. |
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| Catalogue Number | 71346 |
| Brand Family | Novagen® |
| References |
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| Product Information | |||
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| 0.4 ml | 1 ml | Component | |
| • | 2 × 0.2 ml | 5 × 0.2 ml | Origami™ 2(DE3)pLysS Competent Cells |
| • | 2 × 2 ml | 4 × 2 ml | SOC Medium |
| • | 2 ng | 2 ng | Test Plasmid |
| Guaranteed efficiency | 2 × 10⁶ cfu/µg | ||
| Quality Level | MQ100 | ||
| Applications |
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| Biological Information |
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| Physicochemical Information |
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| Dimensions |
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| Materials Information |
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| Toxicological Information |
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| Safety Information according to GHS |
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| Safety Information |
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| Product Usage Statements |
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| Storage and Shipping Information | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
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| Transport Information |
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| Supplemental Information |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 71346-3CN | 04055977253337 |
| 71346-4CN | 04055977253344 |
Documentation
Origami™ 2(DE3)pLysS Competent Cells - Novagen SDS
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Origami™ 2(DE3)pLysS Competent Cells - Novagen Certificates of Analysis
| Title | Lot Number |
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| 71346 |
Brochure
| Title |
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| Competent Cell Brochure |
User Protocols
| Title |
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| TB009 Competent Cells |
| TB053 Academic and Non-profit Laboratory Assurance Letter |
| TB055 pET System Manual |