Millipore Sigma Vibrant Logo

475911 Myeloperoxidase, Human Polymorphonuclear Leukocytes

A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: MPO

View Products on Sigmaaldrich.com
475911
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
475911-100UGCN
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic ampoule 100 μg
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      OverviewNative, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.
      Catalogue Number475911
      Brand Family Calbiochem®
      SynonymsMPO
      References
      ReferencesNauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
      Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
      Mulligan, M.S., et al. 1992. Br. J. Pharmacol. 107, 1159.
      Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun. 189, 1129.
      Product Information
      CAS number9003-99-0
      Unit of DefinitionOne unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.
      EC number1.11.1.7
      FormLyophilized
      FormulationLyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
      Quality LevelMQ200
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Specific Activity150-200 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      475911-100UGCN 04055977203103

      Documentation

      Myeloperoxidase, Human Polymorphonuclear Leukocytes Certificates of Analysis

      TitleLot Number
      475911

      References

      Reference overview
      Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
      Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
      Mulligan, M.S., et al. 1992. Br. J. Pharmacol. 107, 1159.
      Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun. 189, 1129.

      Citations

      Title
    • Armin Schnabel, Elena Csernok, Jorg Braun, Wolfgang Gross. (2000) Activation of Neutrophils, Eosinophils, and Lymphocytes in the Lower Respiratory Tract in Wegener's Granulomatosis. American Journal of Critical Care Medicine 161, 399-405.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-August-2008 RFH
      SynonymsMPO
      DescriptionNative, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.
      FormLyophilized
      FormulationLyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
      SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      CAS number9003-99-0
      EC number1.11.1.7
      Purity≥95% by SDS-PAGE
      Specific activity150-200 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.
      SolubilityH₂O (volume is lot-specific and can be found on the product label). Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.
      Toxicity Standard Handling
      ReferencesNauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
      Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
      Mulligan, M.S., et al. 1992. Br. J. Pharmacol. 107, 1159.
      Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun. 189, 1129.
      Citation
    • Armin Schnabel, Elena Csernok, Jorg Braun, Wolfgang Gross. (2000) Activation of Neutrophils, Eosinophils, and Lymphocytes in the Lower Respiratory Tract in Wegener's Granulomatosis. American Journal of Critical Care Medicine 161, 399-405.
      		•