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105387 Leishman's eosin methylene blue solution modified

for microscopy

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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105387
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Overview

Replacement Information

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      		 Plastic bottle 500 ml
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      Description
      Catalogue Number105387
      DescriptionLeishman's eosin methylene blue solution modified
      OverviewThe staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright's eosin methylene blue solution and May-Grünwald's eosin methylene blue solution,this ready-to-use solution is used for staining of blood and bone marrow smears and clinical cytological specimens. The typical resulting color of cell nuclei is red to purple, which depends on the buffer tablets (Buffer tablets pH 7.2 or Buffer tablets pH 6.8). The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances.
      A 500 mL bottle of Leishman's eosin methylene blue solution are sufficient for staining up to 1600 slides in Hellendahl cells.As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      References
      Product Information
      HS Code3212 90 90
      Quality LevelMQ400
      Applications
      Applicationfor microscopy
      Biological Information
      Physicochemical Information
      Boiling point65 °C (1013 hPa)
      Density0.79 g/cm3 (20 °C)
      Explosion limit5.5 - 44 %(V) Methanol
      Flash point12 °C
      Ignition temperature455 °C Methanol
      Vapor pressure128 hPa (20 °C)
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Statement(s)H225: Highly flammable liquid and vapour.
      H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
      H317: May cause an allergic skin reaction.
      H370: Causes damage to organs.
      Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
      P233: Keep container tightly closed.
      P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
      P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
      P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
      P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
      P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
      Signal WordDanger
      Storage class3 Flammable liquids
      WGKWGK 2 obviously hazardous to water
      Disposal1
      Strongly contaminated halogen-free organic solvents: container A.
      Safety Information
      Categories of dangerhighly flammable, toxic
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +15°C to +25°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
      Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
      Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
      Supplemental Information
      Specifications
      Suitability for microscopy (Blood smear)passes test
      Erythrocytesreddish
      Nucleiviolet
      Eosinophilic granulesbrick-red
      Neutrophilic granuleslight violet
      Basophilic granulesdark violet
      Lymphocyte cytoplasmnblue
      Monocyte cytoplasmgrey-blue
      Thrombocytesviolet
      Global Trade Item Number
      Catalogue Number GTIN
      1.05387.0500 04022536063911

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