Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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402176
MilliporeImmunoPen™
This ImmunoPen™ is validated for use in Frozen Sections, Immunostaining Barrier, Paraffin Sections.
More>>This ImmunoPen™ is validated for use in Frozen Sections, Immunostaining Barrier, Paraffin Sections. Less<<
ImmunoPen™ MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Designed to minimize waste of antibody in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. When applied in a circle around the specimen, forms a hydrophobic barrier, retaining antibody. Sufficient for 500-800 individual applications. Not available for sale in Japan.
To start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip.
Paraffin Section Method: Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed. Frozen Section Method: Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+15°C to +30°C
Do not freeze
Ok to freeze
Special Instructions
Keep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Designed to minimize antibody waste in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. The pen contains liquid resistant to water, alcohol, and acetone. When applied in a circle around the specimen the liquid forms a hydrophobic barrier, retaining antibody and minimizing waste of valuable reagents. Multiple sections, separated by individ-ual circular barriers, can be applied to the same slide. The ImmunoPen™ can be used for immunostaining procedures employing the following methods: PAP (peroxidase-antiperoxidase), ABC, enzymatic, and frozen section. While the barrier is insoluble in alcohol and acetone, it can be removed by xylene after the staining procedure is complete.
Comments
To start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip.
Paraffin Section Method: Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed. Frozen Section Method: Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
Storage
+15°C to +30°C
Do Not Freeze
Ok to freeze
Special Instructions
Keep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.
