Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Description
Overview
Native hyaluronidase from bovine testes. Hyaluronic acid degrading endoglycosidase that catalyzes the hydrolysis of 1,4 linkages between N-acetylhexosamine and D-glucuronate residues in chondroitin, chondroitin-4-sulfate, chondroitin-6-sulfate, and hyaluronic acid.
Note: 1 KU = 1000 units.
Catalogue Number
385931
Brand Family
Calbiochem®
References
References
Lokeshwar, V.B., et al. 2002. J. Biol. Chem.277, 33654. Humphrey, I.H. 1957. Bull. WHO16, 291.
Product Information
CAS number
37326-33-3
Activity
≥2500 units/mg dry weight
Unit of Definition
One unit is defined as the amount of enzyme that will cause the same turbidity reduction as 1.0 unit of International Standard preparation.
Hyaluronidase, Bovine Testes Certificates of Analysis
Title
Lot Number
385931
References
Reference overview
Lokeshwar, V.B., et al. 2002. J. Biol. Chem.277, 33654. Humphrey, I.H. 1957. Bull. WHO16, 291.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-April-2008 JSW
Description
Native hyaluronidase from bovine testes. Catalyzes the hydrolysis of 1,4 linkages between N-acetylhexosamine and D-glucuronate residues in chondroitin, chondroitin-4-sulfate, chondroitin-6-sulfate, and hyaluronic acid.
Form
Lyophilized
CAS number
37326-33-3
EC number
3.2.1.35
Activity
≥2500 units/mg dry weight
Unit definition
One unit is defined as the amount of enzyme that will cause the same turbidity reduction as 1.0 unit of International Standard preparation.
Solubility
Reconstitute in 20 mM sodium phosphate, pH 6.9, 0.45% NaCl, and 0.01% BSA.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 week at 4°C.
Toxicity
Standard Handling
Merck USA index
14, 4758
References
Lokeshwar, V.B., et al. 2002. J. Biol. Chem.277, 33654. Humphrey, I.H. 1957. Bull. WHO16, 291.
