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70796 His•Tag® Monoclonal Antibody

Sensitive, specific detection of His•Tag fusion proteins

His•Tag® Monoclonal Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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70796
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Overview

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70796-3CN
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      		 Plastic ampoule 100 μg
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      70796-4CN
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          		 Plastic ampoule 3 μg
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          Description
          Overview

          The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of the Novagen® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10-8 to 1 × 10-9 M) enables sensitive, specific detection of His•Tag fusion proteins at antibody concentrations of 0.1-0.2 µg/ml. The His•Tag Monoclonal Antibody binds to N-terminal, C-terminal, and internal His•Tag sequences. This antibody also detects the recombinant marker bands in the Perfect Protein™ and Trail Mix™ Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-µg package size provides enough purified antibody for 1000 ml of working solution.

          * Not recommended for HRP-based detection of Trail Mix Western Markers.
          Catalogue Number70796
          Brand Family Novagen®
          References
          Product Information
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          70796-3CN 04055977272512
          70796-4CN 04055977272529

          Documentation

          His•Tag® Monoclonal Antibody SDS

          Title

          Safety Data Sheet (SDS) 

          His•Tag® Monoclonal Antibody Certificates of Analysis

          TitleLot Number
          70796

          Citations

          Title
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        • Kyoung Joon Oh, et al. (2005) Conformational changes in BID, a pro-apoptotic BCL-2 family member, upon membrane binding: a site-directed spin labeling study. Journal of Biological Chemistry 280, 753-767.
        • Kazuki Okamoto and Fumihide Isohashi. (2005) Macromolecular-translocation inhibitor-II (Zn2+-binding protein, parathymosin) interacts with the glucocorticoid receptor and enhances transcription in vivo. Journal of Biological Chemistry 280, 36986-36993.
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        • Tania A. Wong, et al. (2005) Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi. Proceedings of the National Academy of Sciences (USA) 102, 12777-12782.
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        • Carlos Gorbea, et al. (2004) Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles. Journal of Biological Chemistry 279, 54849-54861.
        • Lei Jin, et al. (2004) The three-dimensional structure of the ZAP-70 kinase domain in complex with staurosporine implications for the design of selective innhibitors. Journal of Biological Chemistry 279, 42818-42825.
        • Yuanhe Li, et al. (2004) Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes. European Molecular Biology Organization Journal 23, 2723-2733.
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          User Protocols

          Title
          TB283 His•Tag® Monoclonal Antibody