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341631 Fibronectin, Bovine Plasma

Fibronectin, Bovine Plasma MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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341631
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341631-5MGCN
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      Description
      OverviewNative fibronectin purified from pooled bovine plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
      Catalogue Number341631
      Brand Family Calbiochem®
      References
      Product Information
      CAS number86088-83-7
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3.
      Quality LevelMQ100
      Applications
      Biological Information
      Puritysingle band by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      341631-5MGCN 04055977194906

      Documentation

      Fibronectin, Bovine Plasma Certificates of Analysis

      TitleLot Number
      341631
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-December-2010 RFH
      DescriptionNative fibronectin purified from pooled bovine plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Protocol for Coating of Tissue Culture Plates with Fibronectin

      This protocol is provided only as a guideline; optimal conditions should be determined as needed. This procedure is based on the use of 21 cm2 dishes and 1 mg fibronectin, which is a sufficient quantity to coat 10 dishes at 5 µg/cm2.

      1. Thaw the fibronectin by placing the vial in a 37°C water bath until it is completely thawed. Be VERY careful during the thawing process. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.

      2. After thawing, bring the solution to a final volume of 20 ml with sterile, serum-free medium that has been pre-heated to 37°C. This yields a fibronectin solution of 50 µg/ml.

      3. Add 2 ml to each of 10 tissue culture dishes and swirl gently to completely coat the entrie growth surface.

      4. Incubate the dishes at room temperature for ~45 min to permit binding of the fibronectin to the growth surfaces.

      5. Tilt each dish and remove the fibronecting using a sterile pipette. Do not permit the pipette tip to disturb the growth surface.

      6. Add the cell suspension in medium directly to the fibronectin-coated dishes and incubate under conditions appropriate to the cells.
      CAS number86088-83-7
      Puritysingle band by SDS-PAGE
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      Merck USA index14, 4075