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324725 Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli

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324725
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324725-700MIUCN
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      Glass bottle 700 miu
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      Description
      OverviewRecombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.
      Note: 1 mU = 1 milliunit.
      Catalogue Number324725
      Brand Family Calbiochem®
      SynonymsEndo-β-N-acetylglucosaminidase F1, Endo F1
      References
      ReferencesTarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
      Product Information
      Activity≥17 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5.
      EC number3.2.1.96
      FormLiquid
      FormulationIn 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Biological Information
      Specific Activity≥16 units/mg protein
      Physicochemical Information
      ContaminantsProteases: none detected.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      324725-700MIUCN 04055977215991