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233155 Cleland’s Reagent - CAS 3483-12-3 - Calbiochem

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Dithiothreitol, DTT

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233155
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
3483-12-3C₄H₁₀O₂S₂

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233155-100GMCN
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      233155-1KGCN
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          233155-25GMCN
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              Description
              OverviewA protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.
              Oxidized dithiothreitol: ≤0.5%.
              Catalogue Number233155
              Brand Family Calbiochem®
              SynonymsDithiothreitol, DTT
              References
              ReferencesGailis, L. 1994. Eur. J. Pharmacol. 292, 39.
              Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
              Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
              Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
              Cleland, W.W. 1964. Biochemistry 3, 480.
              Product Information
              CAS number3483-12-3
              FormWhite to off-white powder
              Hill FormulaC₄H₁₀O₂S₂
              Chemical formulaC₄H₁₀O₂S₂
              Quality LevelMQ100
              Applications
              Biological Information
              Purity≥97% by titration
              Physicochemical Information
              ContaminantsHeavy metals: ≤1 ppm
              Heavy metals<1 ppm
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSEK1610000
              Safety Information
              R PhraseR: 22-36/38

              Harmful if swallowed.
              Irritating to eyes and skin.
              S PhraseS: 36-26

              Wear suitable protective clothing.
              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Harmful
              Storage +2°C to +8°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
              Packaging Information
              Packaged under inert gas Packaged under inert gas
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              Catalogue Number GTIN
              233155-100GMCN 07790788054274
              233155-1KGCN 04055977217681
              233155-25GMCN 04055977217711

              Documentation

              Cleland’s Reagent - CAS 3483-12-3 - Calbiochem SDS

              Title

              Safety Data Sheet (SDS) 

              Cleland’s Reagent - CAS 3483-12-3 - Calbiochem Certificates of Analysis

              TitleLot Number
              233155

              References

              Reference overview
              Gailis, L. 1994. Eur. J. Pharmacol. 292, 39.
              Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
              Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
              Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
              Cleland, W.W. 1964. Biochemistry 3, 480.

              Brochure

              Title
              Bulk Product Guide
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision29-April-2008 RFH
              SynonymsDithiothreitol, DTT
              DescriptionDTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL™ Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.
              FormWhite to off-white powder
              Intert gas (Yes/No) Packaged under inert gas
              CAS number3483-12-3
              RTECSEK1610000
              Chemical formulaC₄H₁₀O₂S₂
              Purity≥97% by titration
              ContaminantsHeavy metals: ≤1 ppm
              SolubilityEthanol or H₂O (30 mg/ml)
              Storage +2°C to +8°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
              Toxicity Harmful
              Merck USA index14, 3376
              ReferencesGailis, L. 1994. Eur. J. Pharmacol. 292, 39.
              Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
              Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
              Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
              Cleland, W.W. 1964. Biochemistry 3, 480.