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17-615 ChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set

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17-615
125 µg  25 assays per set. ~5 μg per chromatin immunoprecipitation
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      Overview

      Key Spec Table

      Species ReactivityKey Applications
      H, M, TWB, ChIP
      Description
      Catalogue Number17-615
      Brand Family Upstate
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ Acetyl-Histone H3 set contains purified rabbit polyclonal antibody and the normal rabbit IgG antibody, which can be used to demonstrate that the acetyl-histone H3 antibody is capable of precipitating acetyl-histone H3 associated chromatin. The qPCR primers included flank the human GAPDH promoter and produce a 166 base pair PCR product.
      Alternate Names
      • H3Ac
      • Histone H3 acetyl
      • Histone H3Ac
      Background InformationCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
      Product Information
      FormatPurified
      Control
      • Included negative control antibody rabbit IgG and control primers specific for human GAPDH promoter.
      PresentationAnti-Acetyl-Histone H3 (rabbit polyclonal IgG). One vial containing 125 ug of protein A purified antibody in 125 μL of storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Store at -20°C.

      Normal Rabbit IgG. One vial containing 125 ug of normal rabbit IgG in 125 uL volume. Store at -20°C.

      Control Primers. One vial containing 75 μL of 5 μM of each primer specific for for human GAPDH. Store at -20°C.
      FOR: TAC TAG CGG TTT TAC GGG CG
      REV: TCG AAC AGG AGG AGC AGA GAG
      CGA
      Quality LevelMQ100
      Applications
      ApplicationAcetyl-Histone H3 ChIP validated antibody & primer set including the ChIP-grade antibody & the specific control PCR primers used for chromatin immunoprecipitation of H3Ac.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blot Analysis:
      Acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours (Lane 2) and untreated HeLa cells (Lane 1) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetyl-Histone H3 (0.01 μg/mL).
      Proteins were visualized using a goat-rabbit secondary antibody conjugated to HRP and a chemilumnescence detection system (Please see figures).
      Biological Information
      ImmunogenThe acetyl-histone H3 antibody is made against a peptide corresponding to amino acids 1-20 of Tetrahymena histone H3 ARTKQTAR[K*]STGG[K*]APRKQL(C) where K* is acetylated)
      HostRabbit
      SpecificityRecognizes acetyl-histone H3
      Species Reactivity
      • Human
      • Mouse
      • Tetrahymena
      Species Reactivity NoteProven to react with human and mouse.
      Tetrahymena predicted to cross-react based upon sequence of antibody generating peptide.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight17 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by chromatin immunoprecipitation on HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size125 µg
      Material Package25 assays per set. ~5 μg per chromatin immunoprecipitation
      Global Trade Item Number
      Catalogue Number GTIN
      17-615 04053252588051