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627610 Caspase Inhibitor I - CAS 187389-52-2 - Calbiochem

Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Z-VAD (OMe)-FMK

Primary Target: Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells
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627610
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
187389-52-2C₂₂H₃₀FN₃O₇

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627610-1MGCN
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      627610-5MGCN
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          		 Plastic ampoule 5 mg
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          Description
          OverviewA cell-permeable, irreversible, pan-caspase inhibitor. Inhibits Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells. Shown to enhance the freeze-thaw survival of human embryonic stem cells. When using with purified native or recombinant enzyme, pre-treatment with an esterase is required.
          Catalogue Number627610
          Brand Family Calbiochem®
          SynonymsZ-VAD (OMe)-FMK
          References
          ReferencesHeng, B.C., et al. 2007. Biosci. Rep. 27, 257.
          Martin, U., et al. 2007. Apoptosis 12, 525.
          Misaghi, S., et al. 2004. Chem. Biol. 11, 1677.
          Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
          McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
          Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
          Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
          An, S. and Know, K.A. 1996. FEBS Lett. 386, 115.
          Cain, K., et al. 1996. Biochem J. 314, 27.
          Mirzoeva, O.K., et al. 1996. FEBS Lett. 396, 266.
          Pronk, G.J., et al. 1996. Science 271, 808.
          Slee, E.A., et al. 1996. Biochem. J. 315, 21.
          Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
          Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
          Zhu, H., et al. 1995. FEBS Lett. 374, 303.
          Product Information
          CAS number187389-52-2
          ATP CompetitiveN
          FormWhite solid
          Hill FormulaC₂₂H₃₀FN₃O₇
          Chemical formulaC₂₂H₃₀FN₃O₇
          ReversibleN
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          Biological Information
          Primary TargetFas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells
          PuritySingle spot by TLC
          Physicochemical Information
          Cell permeableY
          Peptide SequenceZ-Val-Ala-Asp(OMe)-CH₂F*
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          627610-1MGCN 04055977262810
          627610-5MGCN 04055977262841

          Documentation

          Caspase Inhibitor I - CAS 187389-52-2 - Calbiochem Certificates of Analysis

          TitleLot Number
          627610

          References

          Reference overview
          Heng, B.C., et al. 2007. Biosci. Rep. 27, 257.
          Martin, U., et al. 2007. Apoptosis 12, 525.
          Misaghi, S., et al. 2004. Chem. Biol. 11, 1677.
          Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
          McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
          Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
          Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
          An, S. and Know, K.A. 1996. FEBS Lett. 386, 115.
          Cain, K., et al. 1996. Biochem J. 314, 27.
          Mirzoeva, O.K., et al. 1996. FEBS Lett. 396, 266.
          Pronk, G.J., et al. 1996. Science 271, 808.
          Slee, E.A., et al. 1996. Biochem. J. 315, 21.
          Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
          Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
          Zhu, H., et al. 1995. FEBS Lett. 374, 303.

          Brochure

          Title
          Caspases and other Apoptosis Related Tools Brochure

          Citations

          Title
        • Heng, B.C., et al. 2007. Biosci. Rep. 27, 257.
        • Naohide Watanabe and Eric Lam. 2005. Journal of Biological Chemistry 280, 14691.
        • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
          		•
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-February-2012 RFH
          SynonymsZ-VAD (OMe)-FMK
          DescriptionA cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.
          FormWhite solid
          CAS number187389-52-2
          Chemical formulaC₂₂H₃₀FN₃O₇
          Peptide SequenceZ-Val-Ala-Asp(OMe)-CH₂F*
          Structure formulaStructure formula
          PuritySingle spot by TLC
          SolubilityDMSO (50 mM)
          Storage Protect from moisture
          -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesHeng, B.C., et al. 2007. Biosci. Rep. 27, 257.
          Martin, U., et al. 2007. Apoptosis 12, 525.
          Misaghi, S., et al. 2004. Chem. Biol. 11, 1677.
          Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
          McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
          Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
          Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
          An, S. and Know, K.A. 1996. FEBS Lett. 386, 115.
          Cain, K., et al. 1996. Biochem J. 314, 27.
          Mirzoeva, O.K., et al. 1996. FEBS Lett. 396, 266.
          Pronk, G.J., et al. 1996. Science 271, 808.
          Slee, E.A., et al. 1996. Biochem. J. 315, 21.
          Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
          Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
          Zhu, H., et al. 1995. FEBS Lett. 374, 303.
          Citation
        • Heng, B.C., et al. 2007. Biosci. Rep. 27, 257.
        • Naohide Watanabe and Eric Lam. 2005. Journal of Biological Chemistry 280, 14691.
        • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
          		•