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14-552 BTK Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant, full-length, human BTK, for use in Kinase Assays.

BTK Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-552
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-552M when ordering the 250 µg size.
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      Overview

      Description
      Catalogue Number14-552
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionBTK Protein, active, 10 µg
      OverviewN-terminal His6-tagged, recombinant, full-length, human BTK
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal His6-tagged, recombinant, full-length, human BTK, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryDefects in the Bruton tyrosine kinase (BTK) gene cause Agammaglobulinemia. Agammaglobulinemia is an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement.
      Gene Symbol
      • BTK
      • PSCTK1
      • MGC126261
      • ATK
      • XLA
      • IMD1
      • AT
      • OTTHUMP00000063593
      • BPK
      • MGC126262
      • AGMX1
      Protein TargetBTK
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q06187 # Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
      COFACTOR: Binds 1 zinc ion per subunit.
      SIZE: 659 amino acids; 76281 Da
      SUBUNIT: Binds GTF2I through the PH domain. Interacts with SH3BP5 via the SH3 domain. Interacts with IBTK via its PH domain. Interacts with GTF2I and ARID3A.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity). Nucleus (By similarity).
      PTM: Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein (By similarity).
      DISEASE: SwissProt: Q06187 # Defects in BTK are the cause of X-linked agammaglobulinemia type 1 (XLA) [MIM:300300]. XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin. & Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA- IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
      SIMILARITY: SwissProt: Q06187 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. & Contains 1 Btk-type zinc finger. & Contains 1 PH domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight78.4kDa
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of Src substrate peptide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-552M when ordering the 250 µg size.
      Global Trade Item Number
      Catalogue Number GTIN
      14-552 04053252512087