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ABE2695-25UG Anti-eIF-4E

Anti-eIF-4E, Cat. No. ABE2695-25UG, is a highly specific rabbit polyclonal antibody, that targets Eukaryotic translation initiation factor 4E and has been tested in Western Blotting and Immunocytochemistry.

Anti-eIF-4E MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: EIF4E, EIF4EL1, EIF4F UniProt Number: P06730
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ABE2695-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2695-25UG
      DescriptionAnti-eIF-4E
      Alternate Names
      • Eukaryotic translation initiation factor 4E
      • eIF4E
      • eIF-4F 25 kDa subunit
      • mRNA cap-binding protein
      Background InformationEukaryotic translation initiation factor 4E (UniProt: P06730; also known as eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein) is encoded by the EIF4E (also known as EIF4EL1, EIF4F) gene (Gene ID: 1977) in human. elF-4E is a cytoplasmic factor that recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. It is a component of the CYFIP1-EIF4E-FMR1 complex that binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex. Three isoforms of elf-4E have been described that are generated by alternative splicing. Elf-4E contains four 7-methylguanosine-containing mRNA cap binding regions and three EIF4EBP1/2/3 binding regions. Hypophosphorylated EIF4EBP1, EIF4EBP2, and EIF4EBP3 are shown to compete with EIF4G1/EIF4G3 to interact with elF-4E. Phosphorylation of ElF4EBP1 by insulin-stimulated MAP kinase (MAPK1 and MAPK3) cause dissociation of the complex and allows EIF4G1/EIF4G3 to bind and result in initiation of translation. Mutations in ElF4E gene have been linked to autism with impairments in social interaction and communication and developmental abnormalities.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-eIF-4E, Cat. No. ABE2695-25UG, is a highly specific rabbit polyclonal antibody, that targets Eukaryotic translation initiation factor 4E and has been tested in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.9 µg/mL from a representative lot detected eIF-4E in 10 µg of PC12 cell lysate.
      Immunocytochemistry Analysis: A 1:100-500 dilution from a representative lot detected eIF-4E in HeLa, A431, HUVEC, and NIH/3T3 cell lines.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Eukaryotic translation initiation factor 4E.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Eukaryotic translation initiation factor 4E in Human, Mouse, and Rat cells. it targets an epitope with in 14 amino acids from the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • EIF4E
      • EIF4EL1
      • EIF4F
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight31 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.
      Western Blotting Analysis: 0.5 µg/mL of this antibody detected eIF-4E in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2695-25UG 04054839464744