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MABE1785 Anti-Ubiquitinated Histone H2B (Lys123) Antibody, clone 1B3F12/A9

Anti-Ubiquitinated Histone H2B (Lys123), clone 1B3F12/A9 Antibody, Cat. No. MABE1785, is a highly specific mouse monoclonal antibody, that targets ubiquitinated Histone H2B and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HTB2, H2B2 UniProt Number: P02293 and P02294
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MABE1785
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, YeastWB, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1785
      DescriptionAnti-Ubiquitinated Histone H2B (Lys123) Antibody, clone 1B3F12/A9
      Alternate Names
      • Histone H2B K123 Ub1
      • Histone H2B.2 UbK123
      Background InformationHistone H2B.2 (UniProt: P02294) is encoded by the HTB2 (also known as H2B2) gene (Gene ID: 852284) in Saccharomyces cerevisiae (yeast). Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Histone H2B is phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase and may be correlated with chromosome condensation. Histone H2B can undergo acetylation by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Ubiquitinated Histone H2B (Lys123), clone 1B3F12/A9 Antibody, Cat. No. MABE1785, is a highly specific mouse monoclonal antibody, that targets ubiquitinated Histone H2B and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected Ubiquitinated Histone H2B (Lys123) in wild type whole cell yeast extracts and ubp8 knockout whole cell yeast extracts.

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Ubiquitinated Histone H2B (Lys123) in H2BK123ub relative to H2B at the coding sequence of the SPA2 gene in budding yeast (relative to WT) (Courtesy of Fred van Leeuwen, Ph.D., Division of Gene Regulation B4, The Netherlands Cancer Institute Plesmanlaan 121, Amsterdam Netherlands).
      Biological Information
      ImmunogenConjugated linear peptide corresponding to 16 amino acids from the C-terminal region of yeast Histone H2B ubiquitinated at Lysine 123.
      Clone1B3F12/A9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1B3F12/A9 detects K123 ubiquitinated histone H2B in human and yeast. It targets an epitope within 16 amino acids from the C-terminal region of ubiquitinated yeast Histone H2B.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Yeast
      Species Reactivity NoteHuman, Yeast
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HTB2
      • H2B2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~23 kDa observed; 14.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell acid extract.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Ubiquitinated Histone H2B (Lys123) in HeLa cell acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1785 04054839396601

      Documentation

      Anti-Ubiquitinated Histone H2B (Lys123) Antibody, clone 1B3F12/A9 Certificates of Analysis

      TitleLot Number
      Anti-Ubiquitinated Histone H2B (Lys123), clone 1B3F12/A9 - 3827413 3827413
      Anti-Ubiquitinated Histone H2B (Lys123), clone 1B3F12/A9 - 3926380 3926380
      Anti-Ubiquitinated Histone H2B (Lys123), clone 1B3F12/A9 - 4202617 4202617
      Anti-Ubiquitinated Histone H2B (Lys123), clone 1B3F12/A9 Monoclonal Antibody Q2952274
      Anti-Ubiquitinated Histone H2B (Lys123), clone 1B3F12/A9 Monoclonal Antibody 3054842