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MAB1222 Anti-Tryptase Antibody, Mast Cell, clone G3

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MAB1222
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1222
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tryptase Antibody, Mast Cell, clone G3
      Background InformationTryptase is the most abundant secretory granule-derived serine proteinase contained in mast cells. Tryptase has recently been used as a marker for mast cell activation and is involved with allergic response. Tryptase may act as a mitogen for fibroblast lines. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis.
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • Mast cells, basophils
      • Human fetal skin lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      (see product datasheet for specific buffer formulation)
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Tryptase Antibody, Mast Cell, clone G3 is validated for use in FC, IH, WB for the detection of Tryptase.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Western Blotting
      Application NotesFlow Cytometry Analysis: A previous lot of this antibody has been used on fixed cells in FACS using a conjugated secondary antibody. (Sperr, W., et al., 2001).

      Optimal working dilutions must be determined by end user.
      Biological Information
      CloneG3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
      Gene Symbol
      • TPSAB1
      • tryptase-I
      • TPS2
      • Tryptase-1
      • TPSB1
      • TPS1
      • tryptase-III
      • EC 3.4.21.59
      • EC 3.4.21.59
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15157 # Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.| Q15661 # Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
      SIZE: 275 amino acids; 30773 Da
      SUBUNIT: Homotetramer.
      SUBCELLULAR LOCATION: Secreted. Note=Released from the secretory granules upon mast cell activation.
      DOMAIN:SwissProt: P15157
      SIMILARITY: SwissProt: P15157 ## Belongs to the peptidase S1 family. Tryptase subfamily. & Contains 1 peptidase S1 domain. | Q15661 ## Belongs to the peptidase S1 family. Tryptase subfamily. & Contains 1 peptidase S1 domain.
      Molecular Weight31 kDa
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on human fetal skin lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected tryptase in 10 µg of human fetal skin lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2–8°C for the duration listed on the product datasheet. (See product datasheet for storage conditions)
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1222 08436037124457