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PC176 Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb

This Anti-Serotonin 2A Receptor Rabbit pAb is validated for use in Frozen Sections, WB, Free Floating Sections, Paraffin Sections for the detection of Serotonin 2A Receptor.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC176
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
M, R, ShRbPolyclonal Antibody

Pricing & Availability

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PC176-100ULCN
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      Description
      OverviewRecognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
      Catalogue NumberPC176
      Brand Family Calbiochem®
      Application Data
      Detection of rat serotonin 2A receptor by immunohistochemistry. Sample: Rat cortex, amygdala, and hippocampus. Primary antibody: Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb (Cat. No. PC176) (1:500). Detection: fluorescence.
      References
      ReferencesLeonard, B.E. 1996. Psychother. Psychosom. 65, 66.
      Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesFree Floating Sections Touri, F., et al. 2004. J. Chem. Neuroanatomy 27, 99. Haszprunar, G., et al. 2002. J. Morphol. 251, 210. Paraffin Sections Jian, B., et al. 2002. Am. J. Pathol. 161, 2111.
      Key Applications Free-floating Sections
      Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (1:300-1:500)
      Immunoblotting (1:500-1:1000)
      Free Floating Sections (see application references)
      Paraffin Sections (see application references; heat pre-treatment required)
      Application CommentsAntibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 22-41 of rat 5-HT2A receptor
      ImmunogenRat
      HostRabbit
      Species Reactivity
      • Mouse
      • Rat
      • Sheep
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC176-100ULCN 04055977209518

      Documentation

      Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC176

      References

      Reference overview
      Leonard, B.E. 1996. Psychother. Psychosom. 65, 66.
      Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-August-2007 JSW
      ApplicationFrozen Sections (1:300-1:500)
      Immunoblotting (1:500-1:1000)
      Free Floating Sections (see application references)
      Paraffin Sections (see application references; heat pre-treatment required)
      Application Data
      Detection of rat serotonin 2A receptor by immunohistochemistry. Sample: Rat cortex, amygdala, and hippocampus. Primary antibody: Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb (Cat. No. PC176) (1:500). Detection: fluorescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
      BackgroundAt least 14 mammalian serotonin receptor subtypes have been identified and classified into several families on the basis of common structural, pharmacological, and functional criteria. Serotonin (5-hydroxytryptamine [5-HT]) mediates its effects in a number of physiological processes including anxiety, depression, sexual activity, and sleep through interactions with different receptor subtypes. These receptors differ in their tissue and cellular localization, affinity for serotonin, and second messenger pathways. The majority of these receptors stimulate a GTP-binding protein upon agonist stimulation and couple to adenylate cyclase or phospholipase C. In contrast, the serotonin 5-HT3 receptor acts as a cation-selective channel. The serotonin receptors have generated considerable pharmacological interest as targets for the identification of selective drugs that interact with a specific receptor subtype.
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide corresponding to amino acids 22-41 of rat 5-HT2A receptor
      Speciesmouse, rat, sheep
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.
      Preservative≤0.1% sodium azide
      CommentsAntibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesLeonard, B.E. 1996. Psychother. Psychosom. 65, 66.
      Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
      Application referencesFree Floating Sections Touri, F., et al. 2004. J. Chem. Neuroanatomy 27, 99. Haszprunar, G., et al. 2002. J. Morphol. 251, 210. Paraffin Sections Jian, B., et al. 2002. Am. J. Pathol. 161, 2111.