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ABF125 Anti-S100A8/MRP-8 Antibody (Azide Free)

This Anti-S100A8/MRP-8 Antibody (Azide Free) is validated for use in Western Blotting and Immunohistochemistry and Activity Assay for the detection of S100A8/MRP-8 (Azide Free).

Anti-S100A8/MRP-8 Antibody (Azide Free) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: S100a8, Caga, Mrp8 UniProt Number: P27005
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ABF125
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHC, ACTRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABF125
      DescriptionAnti-S100A8/MRP-8 Antibody (Azide Free)
      Alternate Names
      • Protein S100-A8
      • Calgranulin-A
      • Chemotactic cytokine CP-10
      • Leukocyte L1 complex light chain
      • Migration inhibitory factor-related protein 8
      • MRP-8
      • p8
      • Pro-inflammatory S100 cytokine
      • S100 calcium-binding protein A8
      • S100A8/MRP-8
      Background InformationS100A8, also known as Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen (CFAG), Leukocyte L1 complex light chain, Migration inhibitory factor related protein 8 (MRP-8), p8, S100 calcium binding protein A8, or Urinary stone protein band A, and encoded by the gene S100A8/CAGA/CFAG/MRP8, is an important protein that binds both zinc and calcium and plays an important role in the regulation of inflammatory processes and immune response. Found in complex with its partner S100A9, S100A8 facilitates chemotaxis, fatty acid trafficking, cytoskeleton reorganization, and NAPDPH oxidase activation and other intracellular activities, as well as a host of extracellular induced activities such as extracellular proinflammatory, antimicrobial, oxidant scavenging, and apoptotic activities . Additionally S100A8 can act as a potent autoimmunity amplifier and appears to augment various cancers and their spread when over expressed. S100A8 is widely expressed and used as biomarker in patients with inflammatory diseases and as a cancer marker in multiple forms of cancer.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-S100A8/MRP-8 Antibody (Azide Free) is validated for use in Western Blotting and Immunohistochemistry and Activity Assay for the detection of S100A8/MRP-8 (Azide Free).
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Activity Assay
      Application NotesActivity Assay Analysis: A representative lot detected that S100A8/MRP-8 reduces mouse neutrophil accumulation in dorsum-raised air pouchs in the presence of MSU crystals (Ryckman, C., et al. (2003). ARTHRITIS & RHEUMATISM. 48(8):2310–2320).
      Immunohistochemistry Analysis: A representative lot detected S100A8/MRP-8 in mouse arthritic paw tissue (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e4578).
      Biological Information
      ImmunogenRecombinant protein corresponding to mouse S100A8/MRP-8.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • S100a8
      • Caga
      • Mrp8
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~10 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human spleen tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected S100A8/MRP-8 in 10 µg of human spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABF125 04053252983405

      Documentation

      Anti-S100A8/MRP-8 Antibody (Azide Free) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-S100A8/MRP-8 Antibody (Azide Free) Certificates of Analysis

      TitleLot Number
      Anti-S100A8/MRP-8 (Azide Free) - Q2441541 Q2441541
      Anti-S100A8/MRP-8 (Azide Free) - 3587082 3587082

      References

      Reference overviewPub Med ID
      Role of S100A8 and S100A9 in neutrophil recruitment in response to monosodium urate monohydrate crystals in the air-pouch model of acute gouty arthritis.
      Ryckman, Carle, et al.
      Arthritis Rheum., 48: 2310-20 (2003)  2003

      Show Abstract       		12905486 12905486