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AB3704 Anti-Orexin-A Antibody

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AB3704
50 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIHCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB3704
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Orexin-A Antibody
      Product Information
      FormatSerum
      PresentationRabbit serum. Lyophilized. Reconstitute with 50 mL of sterile distilled water. Centrifuge to remove any insoluble material. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationDetect Orexin-A using this Anti-Orexin-A Antibody validated for use in IH.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:1,000-1:2,000. The antibody shows intense labeling of Orexin-A neurons with low background.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the C-terminal portion of the bovine Orexin-A peptide.
      HostRabbit
      SpecificityOrexin-A.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a hypothalamic neuropeptide precusor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.
      Gene Symbol
      • HCRT
      • Hypocretin
      • OX
      • orexin
      • Hcrt
      • PPOX
      • PPORX
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43612 # Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
      SIZE: 131 amino acids; 13363 Da
      SUBCELLULAR LOCATION: Rough endoplasmic reticulum (By similarity). Cytoplasmic vesicle (By similarity). Cell junction, synapse (By similarity). Note=Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses (By similarity).
      TISSUE SPECIFICITY: Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
      PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides.
      DISEASE: SwissProt: O43612 # Defects in HCRT are a cause of narcolepsy [MIM:161400]. Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed.
      SIMILARITY: SwissProt: O43612 ## Belongs to the orexin family.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material frozen at -20°C to -70°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1, ACS or better grade) can be added for additional stability.
      Packaging Information
      Material Size50 µL
      Global Trade Item Number
      Catalogue Number GTIN
      AB3704 04053252589218