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487923 Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3)

This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.

Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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487923
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Pricing & Availability

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487923-50UGCN
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      		 Plastic ampoule 50 μg
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      Description
      OverviewRecognizes nitrotyrosine.
      Catalogue Number487923
      Brand Family Calbiochem®
      Application Data
      Detection of nitrotyrosine by immunoblotting. Samples: Nitrotyrosine BSA (lane 1) and whole cell lysate from murine macrophages treated with peroxynitrite (lane 2). Primary antibody: Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) (Cat. No. 487923) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesMyatt, L., et al. 1996. Hypertension 28, 488.
      Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, 0.02% sodium azide, pH 7.2.
      Positive controlMouse macrophages treated with peroxynitrite
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (2 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenperoxynitrite-treated KLH
      CloneCC22.8C7.3
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      487923-50UGCN 04055977200379

      Documentation

      Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) Certificates of Analysis

      TitleLot Number
      487923

      References

      Reference overview
      Myatt, L., et al. 1996. Hypertension 28, 488.
      Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.

      Citations

      Title
    • Matthew Whiteman, Jia Ling Siau and Barry Halliwell. (2003) Lack of Tyrosine Nitration by Hypochlorous Acid in the Presence of Physiological Concentrations of Nitrite. Journal of Biological Chemistry 278, 8380-8384.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-February-2020 JSW
      ApplicationImmunoblotting (2 µg/ml)
      Application Data
      Detection of nitrotyrosine by immunoblotting. Samples: Nitrotyrosine BSA (lane 1) and whole cell lysate from murine macrophages treated with peroxynitrite (lane 2). Primary antibody: Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) (Cat. No. 487923) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.
      BackgroundNitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by Nitric oxide synthase. The reaction of NO with superoxide which is often present under conditions of oxidative stress, forms peroxynitrite, a strong oxidant which can nitrosate tyrosine residues on proteins. Nitrotyrosine has been shown to be present in proteins from artherosclerotic lesions of human carotid arteries and also in placenta during pre-eclampsia.
      HostMouse
      Immunogenperoxynitrite-treated KLH
      CloneCC22.8C7.3
      IsotypeIgG2b
      Speciesa broad range of species
      Positive controlMouse macrophages treated with peroxynitrite
      FormLiquid
      FormulationIn PBS, 50% glycerol, 0.02% sodium azide, pH 7.2.
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesMyatt, L., et al. 1996. Hypertension 28, 488.
      Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.
      Citation
    • Matthew Whiteman, Jia Ling Siau and Barry Halliwell. (2003) Lack of Tyrosine Nitration by Hypochlorous Acid in the Presence of Physiological Concentrations of Nitrite. Journal of Biological Chemistry 278, 8380-8384.
      		•