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ABC61 Anti-NIPP-1 Antibody

This NIPP-1 antibody is validated for use in WB & ICC for the detection of the NIPP-1 protein.

Anti-NIPP-1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: PPP1R8, ARD1, NIPP1 UniProt Number: Q12972
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ABC61
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC61
      DescriptionAnti-NIPP-1 Antibody
      Alternate Names
      • Nuclear inhibitor of protein phosphatase 1
      • NIPP-1
      • Protein phosphatase 1 regulatory inhibitor subunit 8
      • ARD-1
      Background InformationAlso known as ARD1 (Activator of RNA decay), NIPP-1 (nuclear inhibitor of protein phosphatase 1) is a ubiquitously expressed multifunctional nuclear scaffold protein of 39 kDa, which plays an important role in integrating transcription, RNA processing, and cell cycle progression. NIPP-1 serves as a signaling hub in the nucleus. Additionally, NIPP-1 contains at least two binding sites for PP1 and is complexed to about one-third of the nuclear pool of PP1. Normally, the NIPP-1 - PP1 complex is inactive, but can be activated following phosphorylation of NIPP-1 with PKA, CFK2, or Src family kinases. NIPP-1 also interacts directly with nucleic acids and components of polycomb repressive complexe 2 (PRC2) and functions as a PRC2-dependent transcriptional repressor.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Raji nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationThis NIPP-1 antibody is validated for use in WB & ICC for the detection of the NIPP-1 protein.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NIPP-1 in Raji cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human NIPP-1.
      HostRabbit
      SpecificityThis antibody should recognize the alpha and beta isoforms of NIPP-1.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PPP1R8
      • ARD1
      • NIPP1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~42 kDa observed. Three isoforms at 39 kDa (alpha isoform), 23 kDa (beta isoform), and 13 kDa (gamma isoform) are known to exist. An uncharacterized band may be observed at ~62 kDa in some nuclear extracts.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot Analysis in Raji nuclear extract.

      Western Blot Analysis: A 1:1,000 dilution from a representative lot detected NIPP-1 in 10 µg of Raji nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC61 04053252850752