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NE1019
Sigma-AldrichAnti-Myelin Basic Protein Mouse mAb (SMI-99)
This Anti-Myelin Basic Protein Mouse mAb (SMI-99) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections for the detection of Myelin Basic Protein.
More>>This Anti-Myelin Basic Protein Mouse mAb (SMI-99) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections for the detection of Myelin Basic Protein. Less<<
Anti-Myelin Basic Protein Mouse mAb (SMI-99) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes the ~21 kDa myelin basic protein in rat brain tissue. Does not recognize the 14 kDa form. Recognizes most mammalian species.
Catalogue Number
NE1019
Brand Family
Calbiochem®
Application Data
Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
References
References
Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods72, 197. Shin, R.W., et al. 1991. Lab. Invest.64, 693.
Recognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
human myelin basic protein
Immunogen
Human
Epitope
Within amino acids 131-136
Clone
SMI-99
Host
Mouse
Isotype
IgG2b
Species Reactivity
Guinea Pig
Mammals
Antibody Type
Monoclonal Antibody
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Anti-Myelin Basic Protein Mouse mAb (SMI-99) Certificates of Analysis
Title
Lot Number
NE1019
References
Reference overview
Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods72, 197. Shin, R.W., et al. 1991. Lab. Invest.64, 693.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
Description
Purified mouse monoclonal antibody. Recognizes the ~21 kDa myelin basic protein (MBP).
Background
The myelin sheath is essential for rapid and effective propagation of action potential within the axon. Mutations identified in several myelin-related genes reportedly lead to severe demyelinating neuropathies. Recent evidence also
suggests that the autoimmune disease Multiple Sclerosis (MS) is mediated by T cells with specificity for antigens in the myelin sheath. Although myelin in the central nervous system (CNS) and peripheral nervous system (PNS) is structurally similar, it is chemically distinct since oligodendrocytes form myelin in the CNS whereas Schwann cells generate PNS myelin. Myelin proteolipid protein (PLP) is the predominant integral membrane protein in mammalian CNS myelin. In contrast, myelin basic protein (MBP) is localized to the myelin cytoplasm and is highly conserved among species. Both MBP and PLP mediate myelin compaction, whereas thickness is regulated by MBP. Several other myelin proteins have been identified to play distinct or partially overlapping functions in the formation and maintenance of the myelin sheath, including P0, myelin-associated glycoprotein (MAG), peripheral myelin protein (PMP22), and Connexin 32 (Cx32). The development of the myelin sheath has been a good model for understanding the mechanisms of neuron-glia interaction.
Host
Mouse
Immunogen species
Human
Immunogen
human myelin basic protein
Epitope
Within amino acids 131-136
Clone
SMI-99
Isotype
IgG2b
Species
not chicken, guinea pig (weakly), mammalian, not porcine
Positive control
Rat brain
Form
Liquid
Formulation
In PBS.
Preservative
≤0.1% sodium azide
Comments
Recognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods72, 197. Shin, R.W., et al. 1991. Lab. Invest.64, 693.
