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NE1019 Anti-Myelin Basic Protein Mouse mAb (SMI-99)

This Anti-Myelin Basic Protein Mouse mAb (SMI-99) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections for the detection of Myelin Basic Protein.

Anti-Myelin Basic Protein Mouse mAb (SMI-99) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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NE1019
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Gp, MaMMonoclonal Antibody

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      Description
      OverviewRecognizes the ~21 kDa myelin basic protein in rat brain tissue. Does not recognize the 14 kDa form. Recognizes most mammalian species.
      Catalogue NumberNE1019
      Brand Family Calbiochem®
      Application Data
      Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
      References
      ReferencesEvers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
      Shin, R.W., et al. 1991. Lab. Invest. 64, 693.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlRat brain
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (1:1000)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:1000)
      Frozen Sections (1:1000)
      Paraffin Sections (1:1000)
      Application CommentsRecognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenhuman myelin basic protein
      ImmunogenHuman
      EpitopeWithin amino acids 131-136
      CloneSMI-99
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Guinea Pig
      • Mammals
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NE1019-100ULCN 04055977209884

      Documentation

      Anti-Myelin Basic Protein Mouse mAb (SMI-99) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Myelin Basic Protein Mouse mAb (SMI-99) Certificates of Analysis

      TitleLot Number
      NE1019

      References

      Reference overview
      Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
      Shin, R.W., et al. 1991. Lab. Invest. 64, 693.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-July-2008 JSW
      ApplicationELISA (1:1000)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:1000)
      Frozen Sections (1:1000)
      Paraffin Sections (1:1000)
      Application Data
      Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~21 kDa myelin basic protein (MBP).
      BackgroundThe myelin sheath is essential for rapid and effective propagation of action potential within the axon. Mutations identified in several myelin-related genes reportedly lead to severe demyelinating neuropathies. Recent evidence also suggests that the autoimmune disease Multiple Sclerosis (MS) is mediated by T cells with specificity for antigens in the myelin sheath. Although myelin in the central nervous system (CNS) and peripheral nervous system (PNS) is structurally similar, it is chemically distinct since oligodendrocytes form myelin in the CNS whereas Schwann cells generate PNS myelin. Myelin proteolipid protein (PLP) is the predominant integral membrane protein in mammalian CNS myelin. In contrast, myelin basic protein (MBP) is localized to the myelin cytoplasm and is highly conserved among species. Both MBP and PLP mediate myelin compaction, whereas thickness is regulated by MBP. Several other myelin proteins have been identified to play distinct or partially overlapping functions in the formation and maintenance of the myelin sheath, including P0, myelin-associated glycoprotein (MAG), peripheral myelin protein (PMP22), and Connexin 32 (Cx32). The development of the myelin sheath has been a good model for understanding the mechanisms of neuron-glia interaction.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman myelin basic protein
      EpitopeWithin amino acids 131-136
      CloneSMI-99
      IsotypeIgG2b
      Speciesnot chicken, guinea pig (weakly), mammalian, not porcine
      Positive controlRat brain
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      CommentsRecognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesEvers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
      Shin, R.W., et al. 1991. Lab. Invest. 64, 693.