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NA26 Anti-MSH2 (Ab-1) Mouse mAb (GB12)

This Anti-MSH2 (Ab-1) Mouse mAb (GB12) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of MSH2 (Ab-1).

Anti-MSH2 (Ab-1) Mouse mAb (GB12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Mismatch Repair Protein 2

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NA26
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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NA26-100UGCN
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and colon tissue.
      Catalogue NumberNA26
      Brand Family Calbiochem®
      SynonymsAnti-Mismatch Repair Protein 2
      References
      ReferencesBronner, C. E., et al. 1994. Nature 368, 258.
      Papadopoulos, N., et al. 1994. Science 263, 1625.
      Peltomäki, P. T. 1994. Annals of Medicine 26, 215.
      Fishel, R., et al. 1993. Cell 75, 1027-1038, 1993.
      Leach, F. S., et al. 1993. Cell 75, 1215.
      Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Negative controlLoVo cells
      Positive controlHCT116 or SW480 cells or colon tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (5 µg/ml)
      Immunoblotting (1 µg/ml)
      Immunoprecipitation (1 µg/reaction)
      Paraffin Sections (3 µg/ml, heat pre-treatment required)
      Application CommentsFor paraffin sections, pre-treat with heat for 20 min. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenan N-terminal fragment of human MSH2
      ImmunogenHuman
      CloneGB12
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NA26-100UGCN 04055977227703

      Documentation

      Anti-MSH2 (Ab-1) Mouse mAb (GB12) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MSH2 (Ab-1) Mouse mAb (GB12) Certificates of Analysis

      TitleLot Number
      NA26

      References

      Reference overview
      Bronner, C. E., et al. 1994. Nature 368, 258.
      Papadopoulos, N., et al. 1994. Science 263, 1625.
      Peltomäki, P. T. 1994. Annals of Medicine 26, 215.
      Fishel, R., et al. 1993. Cell 75, 1027-1038, 1993.
      Leach, F. S., et al. 1993. Cell 75, 1215.
      Lindbolm, A., et al. 1993. Nature Genetics 5, 279.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      SynonymsAnti-Mismatch Repair Protein 2
      ApplicationFrozen Sections (5 µg/ml)
      Immunoblotting (1 µg/ml)
      Immunoprecipitation (1 µg/reaction)
      Paraffin Sections (3 µg/ml, heat pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP20 mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.
      BackgroundThe MSH2 gene is one of at least 4 genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma, while germline MSH2 mutations are responsible for ~50% of inherited, non-polyposis colorectal carcinoma (HNPCC). MSH2 encodes a protein of 934 amino acids.
      HostMouse
      Immunogen speciesHuman
      Immunogenan N-terminal fragment of human MSH2
      CloneGB12
      IsotypeIgG₁
      Specieshuman, not mouse
      Positive controlHCT116 or SW480 cells or colon tissue
      Negative controlLoVo cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor paraffin sections, pre-treat with heat for 20 min. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesBronner, C. E., et al. 1994. Nature 368, 258.
      Papadopoulos, N., et al. 1994. Science 263, 1625.
      Peltomäki, P. T. 1994. Annals of Medicine 26, 215.
      Fishel, R., et al. 1993. Cell 75, 1027-1038, 1993.
      Leach, F. S., et al. 1993. Cell 75, 1215.
      Lindbolm, A., et al. 1993. Nature Genetics 5, 279.