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MAB1922Z Anti-Laminin α2 Antibody, clone 5H2, Azide Free

Anti-Laminin α2 Antibody, clone 5H2, Azide Free detects level of Laminin α2 & has been published & validated for use in ELISA, IH, IP & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: LAMA2, LAMM UniProt Number: P24043
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MAB1922Z
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk, RbELISA, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1922Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Laminin α2 Antibody, clone 5H2, Azide Free
      Alternate Names
      • MerosinMchain
      • MAB1922
      Product Information
      FormatPurified
      Quality LevelMQ100
      Applications
      ApplicationAnti-Laminin α2 Antibody, clone 5H2, Azide Free detects level of Laminin α2 & has been published & validated for use in ELISA, IH, IP & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesELISA: 50% maximal binding to human merosin at 1:50,000.

      Immunohistochemistry: 1:5,000 for staining of 8 mm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.

      Immunofluorescence

      Affinity chromatography

      Immunoprecipitation

      Immunoblotting

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified human merosin.
      Clone5H2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with the 80 kDa fragment of the M-chain of human merosin.

      SPECIES REACTIVITIES:

      Cross reacts with monkey and rabbit merosin.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Monkey
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryLaminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.
      Gene Symbol
      • LAMA2
      • LAMM
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P24043 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
      SIZE: 3110 amino acids; 342771 Da
      SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-2 is a subunit of laminin-2 (merosin) and laminin-4 (S- merosin).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
      TISSUE SPECIFICITY: Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
      DOMAIN: SwissProt: P24043 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI, IV and G are globular.
      DISEASE: SwissProt: P24043 # Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
      SIMILARITY: Contains 17 laminin EGF-like domains. & Contains 5 laminin G-like domains. & Contains 2 laminin IV type A domains. & Contains 1 laminin N-terminal domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain between 2 and 8°C for up to 6 months.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1922Z 04053252663703