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420400 Anti-KDEL Mouse mAb (10C3)

This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.

Anti-KDEL Mouse mAb (10C3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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420400
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ca, Dr, Gp, Ht, H, Mk, M, Porcine, Rb, R, Sh, YeastMMonoclonal Antibody

Pricing & Availability

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      		 Plastic ampoule 50 μg
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      Description
      OverviewRecognizes the Grp78 (BiP) and Grp94 proteins.
      Catalogue Number420400
      Brand Family Calbiochem®
      Application Data
      Detection of KDEL by immunoblotting. Samples: Recombinant Grp78 (BiP) protein (lane 1), whole cell lysate RK-13 cells (lane 2), mouse liver microsomes (lane 3), rat liver microsomes (lane 4), and whole cell lysate from heat shocked HeLa cells (lane 5). Primary antibody: Anti-KDEL Mouse mAb (10C3) (Cat. No. 420400) (1:1000). Detection: chemiluminescence.
      References
      ReferencesKuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990.
      Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
      Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
      Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Positive controlRK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (4 µg/ml, colorimetric)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (5 µg/ml)
      Application CommentsAlso recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH
      ImmunogenRat
      Clone10C3
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Canine
      • Drosophila
      • Guinea Pig
      • Hamster
      • Human
      • Monkey
      • Mouse
      • Porcine
      • Rabbit
      • Rat
      • Sheep
      • Yeast
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      420400-50UGCN 04055977187571

      Documentation

      Anti-KDEL Mouse mAb (10C3) Certificates of Analysis

      TitleLot Number
      420400

      References

      Reference overview
      Kuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990.
      Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
      Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
      Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2007 RFH
      ApplicationImmunoblotting (4 µg/ml, colorimetric)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (5 µg/ml)
      Application Data
      Detection of KDEL by immunoblotting. Samples: Recombinant Grp78 (BiP) protein (lane 1), whole cell lysate RK-13 cells (lane 2), mouse liver microsomes (lane 3), rat liver microsomes (lane 4), and whole cell lysate from heat shocked HeLa cells (lane 5). Primary antibody: Anti-KDEL Mouse mAb (10C3) (Cat. No. 420400) (1:1000). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.
      BackgroundSeveral resident proteins in the ER are involved in the proper assembly and folding of newly synthesized proteins. These include the glucose-regulated protein 94 (Grp94), BiP, which functions in protein oligomerization, and protein disulfide isomerase (PDI), which catalyzes the rearrangement of the disulfide bond. Grp94, BiP, and PDI share a carboxy-terminal sequence (Lys-Asp-Glu-Leu) with other resident ER proteins. The KDEL sequence is sufficient for the retention of a protein in the ER.
      HostMouse
      Immunogen speciesRat
      Immunogena synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH
      Clone10C3
      IsotypeIgG2a
      SpeciesDrosophila, canine, guinea pig, hamster, human, monkey, mouse, porcine, rabbit, rat, sheep, yeast
      Positive controlRK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAlso recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Highly Toxic
      ReferencesKuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990.
      Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
      Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
      Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.