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MABT1501-100UG Anti-JWA Antibody, clone 967C3

MABT1501-100UG
100 μg  
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      Overview

      Description
      Catalogue NumberMABT1501-100UG
      DescriptionAnti-JWA Antibody, clone 967C3
      Alternate Names
      • PRA1 family protein 3
      • ADP-ribosylation factor-like protein 6-interacting protein 5
      • ARL-6-interacting protein 5
      • Aip-5
      • Cytoskeleton-related vitamin A-responsive protein
      • Dermal papilla-derived protein 11
      • GTRAP3-18
      • Glutamate transporter EAAC1-interacting protein
      • JM5
      • Prenylated Rab acceptor protein 2
      • Protein JWa
      • Putative MAPK-activating protein PM27
      Background InformationPRA1 family protein 3 (UniProt: O75915; also known as ADP-ribosylation factor-like protein 6-interacting protein 5, ARL-6-interacting protein 5, Aip-5, Cytoskeleton-related vitamin A-responsive protein, Dermal papilla-derived protein 11, GTRAP3-18, Glutamate transporter EAAC1-interacting protein, JM5, Prenylated Rab acceptor protein 2, Protein JWA, Putative MAPK-activating protein PM27) is encoded by the ARL6IP5 (also known as DERP11, JWA, PRA2, PRAF3) gene (Gene ID: 10550) in human. JWA is a multi-pass membrane protein that also exists in a soluble form in the cytoplasm. It exists as either as a homodimer or as a heterodimer with ARL6IP1. Its homo or heterodimerization requires amino acids sequence 103-117 and another sequence (aa 136-188) is required for its targeting to the endoplasmic reticulum membrane. Its levels are reported to be up-regulated upon induced differentiation and during heat stress. JWA expression is dependent upon generation of intracellular reactive oxygen species (ROS) and it protects cells against ROS-associated DNA damage. JWA serves as a repair protein by regulating base excision repair. It can also serve as a tumor suppressor that can regulate tumor angiogenesis by suppressing the activity of matrix metalloproteinase and inhibiting invasion via focal adhesion kinase. JWA is also known to regulate cancer cells differentiation and apoptosis induced by multiple chemicals. However, its levels are relatively lower in many types of tumors. JWA is shown to sensitize p-glycoprotein-mediated drug resistance to etoposide, a topoisomerase II inhibitor (Ref.: Li, Y., et al. (2015). Sci. Rep. 5; 11009).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-JWA, clone 967C3, Cat. No. MABT1501, is a mouse monoclonal antibody that detects PRA1 family protein 3 (JWA) and has been tested for use in Immunoprecipitation and Western blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected JWA in Western Blotting applications (Li, Y., et. al. (2015). Sci Rep. 5:11009).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated JWA in Immunoprecipitation applications (Wang, R., et. al. (2018). Cell Death Dis. 9(3):352).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human PRA1 family protein 3 (JWA).
      Clone967C3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 967C3 is a mouse monoclonal antibody that detects PRA1 family protein 3 (JWA). It targets an epitope with in 13 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ARL6IP5
      • DERP11
      • JWA
      • PRA2
      • PRAF3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~22 kDa observed; 21.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected JWA in NIH3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1501-100UG 04054839961717