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OP133 Anti-IKKα Mouse mAb (14A231)

This Anti-IKKα Mouse mAb (14A231) is validated for use in Immunoblotting for the detection of IKKα.

Anti-IKKα Mouse mAb (14A231) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP133
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, Mk, MMMonoclonal Antibody

Pricing & Availability

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OP133-100UGCN
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~85 kDa IKKα protein in Daudi cells.
      Catalogue NumberOP133
      Brand Family Calbiochem®
      Application Data
      Detection of human IKKα by immunoblotting. Sample: Whole cell lysate (30 µg) from Daudi cells. Primary antibody: Anti-IKKα Mouse mAb (14A231) (Cat. No. OP133) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesZandi, E., et al. 1998 Science 281, 1360.
      Didonato, J.A., et al. 1997 Nature 388, 548.
      Lee, F.S., et al. 1997 Cell 88, 213.
      Mercurio, F., et al. 1997 Science 278, 860.
      Regnier, C.H., et al. M. 1997 Cell 90, 373.
      Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.2% gelatin, pH 7.4.
      Positive controlDaudi cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-2 µg/ml, chemiluminescence)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag® sequence
      ImmunogenHuman
      Clone14A231
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Monkey
      • Mouse
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP133-100UGCN 04055977224801

      Documentation

      Anti-IKKα Mouse mAb (14A231) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-IKKα Mouse mAb (14A231) Certificates of Analysis

      TitleLot Number
      OP133

      References

      Reference overview
      Zandi, E., et al. 1998 Science 281, 1360.
      Didonato, J.A., et al. 1997 Nature 388, 548.
      Lee, F.S., et al. 1997 Cell 88, 213.
      Mercurio, F., et al. 1997 Science 278, 860.
      Regnier, C.H., et al. M. 1997 Cell 90, 373.
      Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationImmunoblotting (1-2 µg/ml, chemiluminescence)
      Application Data
      Detection of human IKKα by immunoblotting. Sample: Whole cell lysate (30 µg) from Daudi cells. Primary antibody: Anti-IKKα Mouse mAb (14A231) (Cat. No. OP133) (1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified mouse monoclonal antibody. Recognizes the ~85 kDa IKKα protein.
      BackgroundNF-κB (nuclear factor κB) is sequestered in the cytoplasm by IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β and IKK3/γ. In vitro, IKK1/α and IKK2/β can form homo- and heterodimers that can phosphorylate IκBs at the regulatory serine residues directly. IKK1/α and IKK2/β are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK1/α gene in mice results in skin and limb abnormalities and death of newborns.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag® sequence
      Clone14A231
      IsotypeIgG₁
      Specieshuman, monkey, mouse
      Positive controlDaudi cells
      FormLiquid
      FormulationIn PBS, 0.2% gelatin, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesZandi, E., et al. 1998 Science 281, 1360.
      Didonato, J.A., et al. 1997 Nature 388, 548.
      Lee, F.S., et al. 1997 Cell 88, 213.
      Mercurio, F., et al. 1997 Science 278, 860.
      Regnier, C.H., et al. M. 1997 Cell 90, 373.
      Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.