Millipore Sigma Vibrant Logo

AB9900 Anti-IDO Antibody

View Products on Sigmaaldrich.com
AB9900
100 µg  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MELISA, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9900
      Replaces04-1056
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-IDO Antibody
      Alternate Names
      • Indoleamine 2,3-dioxygenase
      Product Information
      FormatPurified
      PresentationLiquid on PBS, pH 7.4.
      Quality LevelMQ100
      Applications
      ApplicationAnti-IDO Antibody detects level of IDO & has been published & validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1:2,000-1:5,000 using ECL. Reacts with the ~45 kDa IDO protein. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.

      ELISA, direct and indirect: 1:2,000-1:10,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant mouse IDO.
      HostRabbit
      SpecificityIDO [Indoleamine 2,3-dioxygenase], mouse.
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]
      Gene Symbol
      • INDO
      • IDO
      • EC 1.13.11.52
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14902 # Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
      COFACTOR: Binds 1 heme group per subunit.
      SIZE: 403 amino acids; 45326 Da
      SIMILARITY: SwissProt: P14902 ## Belongs to the indoleamine 2,3-dioxygenase family.
      Molecular Weight45 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AB9900 04053252367113