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ABT11 Anti-Fibroblast activation protein alpha (FAP) Antibody

Detect Fibroblast activation protein alpha (FAP) using this Anti-Fibroblast activation protein alpha (FAP) Antibody validated for use in WB, IH.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure, Apoptosis & Cancer Research Sub-Category: ECM Proteins, Tumor Markers Gene Symbol: FAPA, FAP, DPPIV UniProt Number: Q12884
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ABT11
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, FUNCRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT11
      DescriptionAnti-Fibroblast activation protein alpha (FAP) Antibody
      Alternate Names
      • fibroblast activation protein, alpha
      • fibroblast activation protein, alpha subunit
      • integral membrane serine protease
      • seprase
      • 170 kDa melanoma membrane-bound gelatinase, FAP
      Background InformationFibroblast activation protein alpha (FAP), also known as Seprase, is a 97 kDa, type II cell surface glycoprotein belonging to the serine protease family. Although FAP is considered fibroblast specific, it is not found in normal healthy fibroblasts. FAP is most commonly found in stromal, tumor fibroblasts, and is likely involved in ECM pericellular proteolysis, endothelial cell migration and invasion into the ECM. The presence of FAP in human epithelial carcinoma is increased, particularly in breast, colorectal, lung, and ovarian carcinomas. FAP is also transiently observed in developing cells, or in tissues that are undergoing wound healing or suffering from chronic inflammatory conditions. Due to its specific distribution both to tumor fibroblasts and to inflammed tissue, FAP makes a promising target for research in tumor-stromal studies and in therapeutic studies.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • HEK-FAP xenograft tumor lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS (pH 7.2) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Fibroblast activation protein alpha (FAP) using this Anti-Fibroblast activation protein alpha (FAP) Antibody validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Affects Function
      Application NotesImmunohistochemistry: A 1:750 dilution of a previous lot of FAP antibody was used to immunohistochemically stain frozen tissue sections of HEK-FAP or HT-29 xenograft tumors from C.B17 mice (Cheng, 2002).

      Inhibition: Inhibition of dipeptidyl peptidase activity with a previous lot of FAP antibody on HT-29 xenograft tumors was demonstrated by an independent laboratory (Cheng, 2002).
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human FAP.
      EpitopeExtracellular domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody recognizes the extracellular domain of Fibroblast activation protein alpha
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. [provided by RefSeq].
      Gene Symbol
      • FAPA
      • FAP
      • DPPIV
      Purification MethodAmmonium Sulfate
      UniProt Number
      UniProt SummaryFUNCTION: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

      CATALYTIC ACTIVITY: Degrades gelatin and heat-denatured type I and type IV collagen, but not native type I or type IV collagen. Does not cleave laminin, fibronectin, fibrin or casein.

      SUBUNIT STRUCTURE: Homodimer, or heterodimer with DPP4. The monomer is inactive.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. Cell projection › lamellipodium membrane; Single-pass type II membrane protein. Cell projection › invadopodium membrane; Single-pass type II membrane protein. Note: Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.

      TISSUE SPECIFICTY: Fibroblast specific.

      INDUCTION: In fibroblasts at times and sites of tissue remodeling during development, tissue repair, and carcinogenesis.

      PTM: N-glycosylated. Ref.11 Ref.9

      The N-terminus may be blocked.

      SEQUENCE SIMILARITIES: Belongs to the peptidase S9B family.
      Molecular Weight~ 90 kDa observed MW. Two isoforms of the Fibroblast activation protein exist at calculated MWs of 88kDa and 27 kDa. It is possible that both bands may be obserbved in certain cell lines.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK-FAP xenograft tumor lysate.

      Western Blot Analysis: 2.5 µg/mL of this antibody detected FAP in 10 µg of HEK-FAP xenograft tumor lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT11 04053252421778

      Documentation

      Anti-Fibroblast activation protein alpha (FAP) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Fibroblast activation protein alpha (FAP) Antibody Certificates of Analysis

      TitleLot Number
      Anti-Fibroblast activation protein alpha (FAP) 3063843
      Anti-Fibroblast activation protein alpha (FAP) 2475938
      Anti-Fibroblast activation protein alpha (FAP) - 2127825 2127825
      Anti-Fibroblast activation protein alpha (FAP) - 2327011 2327011
      Anti-Fibroblast activation protein alpha (FAP) - 1987098 1987098
      Anti-Fibroblast activation protein alpha (FAP) - 2150749 2150749
      Anti-Fibroblast activation protein alpha (FAP) - 2207216 2207216
      Anti-Fibroblast activation protein alpha (FAP) - 2216893 2216893
      Anti-Fibroblast activation protein alpha (FAP) - 2239626 2239626
      Anti-Fibroblast activation protein alpha (FAP) - 2280473 2280473
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