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07-1562 Anti-EZH2 Antibody

Use Anti-EZH2 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EZH2 also known as SET domain bifurcated 1, ERG-associated protein with SET domain.

Anti-EZH2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology, Histones Gene Symbol: EZH2, MGC9169, ENX-1 UniProt Number: Q15910, BC010858
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07-1562
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-1562
      Replaces04-1047
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-EZH2 Antibody
      Alternate Names
      • SETDB1
      • EC 2.1.1.43
        SET domain bifurcated 1
        ERG-associated protein with SET domain
        ESET
        Histone H3-K9 methyltransferase 4
        H3-K9-HMTase 4
        Lysine N-methyltransferase 1E
      Background InformationPolycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro.. EZH2, also known as ENX-1, is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis and placenta. EZH2 and BMI-1 genes are co expressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene.
      References
      Product Information
      FormatSerum
      Control
      • A431 cell lysate
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-EZH2 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EZH2 also known as SET domain bifurcated 1, ERG-associated protein with SET domain.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, His-tagged fusion protein corresponding to residues 406-746 of human EHZ2 (similar to enhancer of zeste (Drosophila) homolog 2). Based on accession # BC010858.
      HostRabbit
      SpecificityRecognizes EZH2, Mr ~100 kDa
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.
      Gene Symbol
      • EZH2
      • MGC9169
      • ENX-1
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15910 # May be involved in the regulation of gene transcription and chromatin structure.
      SIZE: 746 amino acids; 85363 Da
      SUBUNIT: Binds ATRX via the SET domain (Probable).
      SUBCELLULAR LOCATION: Nucleus (Probable).
      TISSUE SPECIFICITY: Expressed in many tissues.
      SIMILARITY: SwissProt: Q15910 ## Belongs to the EZ family. & Contains 1 SET domain.

      Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A
      Molecular Weight95-100kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot analysis
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-1562 04053252671272