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GR13L Anti-EGFR (Ab-3) Mouse mAb (225)

Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.

Anti-EGFR (Ab-3) Mouse mAb (225) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-EGF-R

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GR13L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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GR13L-100UGCN
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.
      Catalogue NumberGR13L
      Brand Family Calbiochem®
      SynonymsAnti-EGF-R
      References
      ReferencesHunter T. 1984. Nature 311, 414.
      Reynolds, F.H., et. al 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
      Negative controlHL-60 cells
      Positive controlA431 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511.
      Key Applications Immunoprecipitation
      Neutralization Studies
      Application NotesImmunoprecipitation (1-2 µg per sample)
      Neutralization Studies (2 µg/ml, see comments and application references)
      Application CommentsThis antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified, human EGFR from A431 cells
      ImmunogenHuman
      Clone225
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GR13L-100UGCN 04055977228236

      Documentation

      Anti-EGFR (Ab-3) Mouse mAb (225) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-EGFR (Ab-3) Mouse mAb (225) Certificates of Analysis

      TitleLot Number
      GR13L

      References

      Reference overview
      Hunter T. 1984. Nature 311, 414.
      Reynolds, F.H., et. al 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-October-2007 JSW
      SynonymsAnti-EGF-R
      ApplicationImmunoprecipitation (1-2 µg per sample)
      Neutralization Studies (2 µg/ml, see comments and application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
      BackgroundEpidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF receptor is phosphorylated on tyrosine residues. Subsequent to binding, EGF receptor complexes are internalized and cell proliferation takes place with partially-purified EGF receptor from human A431 epidermal carcinoma cells.
      HostMouse
      Immunogen speciesHuman
      Immunogenpartially purified, human EGFR from A431 cells
      Clone225
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlA431 cells
      Negative controlHL-60 cells
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
      PreservativeNone
      CommentsThis antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesHunter T. 1984. Nature 311, 414.
      Reynolds, F.H., et. al 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Application referencesOriginal Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies