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MAB8127 Anti-Cytomegalovirus Antibody, late Antigen, clone 1G5.2

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MAB8127
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IF, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB8127
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytomegalovirus Antibody, late Antigen, clone 1G5.2
      Alternate Names
      • CMV
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationDetect Cytomegalovirus using this Anti-Cytomegalovirus Antibody, late antigen, clone 1G5.2 validated for use in FC, IF, IH(P).
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      Applications Not Recommended
      • Western Blotting
      Application NotesImmunochemistry.

      IFA at 1:400-1:800 on acetone fixed cells.

      Works on paraffin embedded tissue sections.

      Dilute with buffer pH 7.4-7.6 to desired working volume.

      For extensive dilution, protein containing or other stabilizing medium should be used.

      Final working dilutions must be determined by end user.
      Biological Information
      ImmunogenSucrose gradient purified CMV AD169 (ATCC).
      Epitopelate antigen
      Clone1G5.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with a late protein. Can detect CMV infection 24 hours post-infection exhibiting a cytoplasmic staining which reaches peak intensity at >72 hours.

      With CMV the antigens expressed at different times are listed as:



      Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

      Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

      Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at +4°C for up to 12 months
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB8127 04053252663970