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AB9826-200UL Anti-Cav3.2 Antibody

Anti-Cav3.2 Antibody detects level of Cav3.2 & has been published & validated for use in WB.

Anti-Cav3.2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters, Signaling Neuroscience Gene Symbol: CACNA1H: CAC1H UniProt Number: Q9EQ60, O95180
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AB9826-200UL
50 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9826-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cav3.2 Antibody
      References
      Product Information
      FormatAffinity Purified
      Control
      • Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cav3.2 Antibody detects level of Cav3.2 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:200 using ECL on ND7/23 cell lysate (mouse neuroblastoma x rat DRG neuron hybrid cell line).
      Dilutions should be made using a carrier protein such as BSA (1-3%).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified peptide from amino acids 581-595 of rat Cav3.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Cav3.2 (α1H (T-Type)).
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteThe immunogen sequence is 14 of 15 amino acids identical in mouse, bovine and canine and 13 of 15 amino acids identical in human.
      Antibody TypePolyclonal Antibody
      Entrez Gene SummaryThis gene encodes a T-type member of the alpha-1 subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell. There are multiple isoforms of each of the proteins in the complex, either encoded by different genes or the result of alternative splicing of transcripts. Alternate transcriptional splice variants, encoding different isoforms, have been characterized for the gene described here. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (CAE).
      Gene Symbol
      • CACNA1H: CAC1H
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O95180 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the low-voltage activated (LVA) group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.
      SIZE: 2353 amino acids; 259163 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Expressed in kidney, liver, heart, brain. Isoform 2 seems to be testis-specific.
      DOMAIN: SwissProt: O95180 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
      PTM: In response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II.
      SIMILARITY: Belongs to the calcium channel alpha-1 subunit family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB9826-200UL 04053252336386

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies