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MABF2031-25UG Anti-CD1b Antibody, clone BCD1b3.1

Anti-CD1b, clone BCD1b3.1, Cat. No. MABF2031, is a mouse monoclonal antibody that detects T-cell surface glycoprotein CD1b and it has been tested for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition assays, and Neutralizing function.

Anti-CD1b Antibody, clone BCD1b3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CD1B UniProt Number: P29016
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MABF2031-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, GpFC, IHC, IP, Inhibition, NEUTMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2031-25UG
      DescriptionAnti-CD1b Antibody, clone BCD1b3.1
      Alternate Names
      • T-cell surface glycoprotein CD1b
      Background InformationT-cell surface glycoprotein CD1b (UniProt: P29016; also known as CD1b) is encoded by the CD1B gene (Gene ID: 910) in human. CD1b is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. The most prominent known function of CD1b molecules is to associate with lipids from pathogens in order to stimulate T cells and induce host defense mechanisms. CD1b is expressed on cortical thymocytes, certain T-cell leukemia, and other tissues. Two isoforms of CD1b are reported that are produced by alternative splicing. CD1b has an extracellular domain (aa 18-3-3), a helical domain (aa 304-324), and a cytoplasmic tail (aa 329-332). The newly synthesized CD1b molecules follow an intracellular transport pathway that leads it to the cell surface from endoplasmic reticulum and subsequently to internalization from the plasma membrane into the endocytic pathway. After internalization, CD1b molecules are sorted for transport to lysosomes in the sorting endosome. (Ref.: Briken, V., et al. (2002). EMBO J. 21(4); 825-834).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationAnti-CD1b, clone BCD1b3.1, Cat. No. MABF2031, is a mouse monoclonal antibody that detects T-cell surface glycoprotein CD1b and it has been tested for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition assays, and Neutralizing function.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Inhibition
      • Neutralizing
      Application NotesImmunohistochemistry Analysis: A representative lot detected CD1b in Immunohistochemistry applications (Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88).

      Flow Cytometry Analysis: A representative lot detected CD1b in Flow Cytometry applications (Hiromatsu, K., et. al. (2002). Immunology. 106(2):159-72; Torrelles, J.B., et. al. (2011). J Biol Chem. 286(41):35438-46; Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88; Sieling, P.A., et. al. (2005). J Immunol. 174(5):2637-44; Facciotti, F., et. al. (2011). Proc Natl Acad Sci USA. 108(34):14228-33).

      Immunoprecipitation Analysis: A representative lot detected CD1b in Immunoprecipitation applications (Briken, V., et. al. (2002). EMBO J. 21(4):825-34; Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88).

      Neutralizing Analysis: A representative lot detected CD1b in Neutralizing applications (Torrelles, J.B., et. al. (2011). J Biol Chem. 286(41):35438-46).

      Inhibition Analysis: A representative lot detected CD1b in Inhibition applications (Behar, S.M., et. al. (1995). J Exp Med. 182(6):2007-18).
      Biological Information
      ImmunogenIsolated human CD1+ monocytes.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone BCD1b3.1 detects T-cell surface glycoprotein CD1b in human and guinea pig.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Guinea Pig
      Species Reactivity NoteGuinea Pig, Human.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • CD1B
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight37 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in MOLT4 cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected CD1b in one million MOLT4 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2031-25UG 04054839476884