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217660 Anti-CD18 Mouse mAb (IB4)

Overview

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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217660-100UGCN
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      Description
      OverviewRecognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.
      Catalogue Number217660
      Brand Family Calbiochem®
      SynonymsAnti-β2 Integrin
      References
      ReferencesBerman, M.E., et al. 1996. J. Immunol. 156, 1515.
      Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
      Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161.
      Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
      Landis, R.C., et al. 1994. J. Cell Biol. 126, 529.
      Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Negative controlNalm-6 cells
      Positive controlHPB-MLT cells
      Preservative0.5 mg/ml gentamicin sulfate
      Quality LevelMQ100
      Applications
      Key Applications Cell Inhibition Assays
      Flow Cytometry
      Frozen Sections
      Immunoprecipitation
      Application NotesFlow Cytometry (5-10 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Cell Inhibition Assays (see comments)
      Application CommentsBlocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman peripheral blood monocytes
      ImmunogenHuman
      CloneIB4
      HostMouse
      Purity≥95% by SDS-PAGE
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Global Trade Item Number
      Catalogue Number GTIN
      217660-100UGCN 04055977202946