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217660
Sigma-AldrichAnti-CD18 Mouse mAb (IB4)
Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.
More>>Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays. Less<<
Anti-CD18 Mouse mAb (IB4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.
Catalogue Number
217660
Brand Family
Calbiochem®
Synonyms
Anti-β2 Integrin
References
References
Berman, M.E., et al. 1996. J. Immunol.156, 1515. Kaneko, M., et al. 1995. J. Immunol. 155, 2631. Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161. Kaneko, M., et al. 1995. J. Immunol.155, 2631. Landis, R.C., et al. 1994. J. Cell Biol.126, 529. Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.
Product Information
Form
Liquid
Formulation
In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Flow Cytometry (5-10 µg/ml) Frozen Sections (see comments) Immunoprecipitation (see comments) Cell Inhibition Assays (see comments)
Application Comments
Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
human peripheral blood monocytes
Immunogen
Human
Clone
IB4
Host
Mouse
Purity
≥95% by SDS-PAGE
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Anti-CD18 Mouse mAb (IB4) Certificates of Analysis
Title
Lot Number
217660
References
Reference overview
Berman, M.E., et al. 1996. J. Immunol.156, 1515. Kaneko, M., et al. 1995. J. Immunol. 155, 2631. Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161. Kaneko, M., et al. 1995. J. Immunol.155, 2631. Landis, R.C., et al. 1994. J. Cell Biol.126, 529. Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-July-2009 JSW
Synonyms
Anti-β2 Integrin
Application
Flow Cytometry (5-10 µg/ml) Frozen Sections (see comments) Immunoprecipitation (see comments) Cell Inhibition Assays (see comments)
Description
Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).
Background
Human CD18 (β2 integrin) is expressed on leukocytes as part of a heterodimeric complex with either CD11a, b or c (integrins αL, αM, or αX). CD18/CD11 heterodimers function in cell adhesion in immune and inflammatory responses.
Host
Mouse
Immunogen species
Human
Immunogen
human peripheral blood monocytes
Clone
IB4
Isotype
IgG2a
Species
human
Positive control
HPB-MLT cells
Negative control
Nalm-6 cells
Form
Liquid
Formulation
In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label
Please refer to vial label for lot-specific concentration
Purity
≥95% by SDS-PAGE
Preservative
0.5 mg/ml gentamicin sulfate
Comments
Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity
Standard Handling
References
Berman, M.E., et al. 1996. J. Immunol.156, 1515. Kaneko, M., et al. 1995. J. Immunol. 155, 2631. Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161. Kaneko, M., et al. 1995. J. Immunol.155, 2631. Landis, R.C., et al. 1994. J. Cell Biol.126, 529. Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.