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AB1031 Anti-Aggrecan Antibody

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AB1031
50 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIF, IH(P), IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1031
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Aggrecan Antibody
      Alternate Names
      • Cartilage-specific proteoglycan core protein
      • Cartilage-specific proteoglycan core protein
      • aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122)
      • aggrecan proteoglycan
      • aggrecan proteoglycan
      • large aggregating proteoglycan
      Background InformationAggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight.
      Product Information
      FormatAffinity Purified
      Control
      • Cartilage, neural tube, and brain tissue.
      PresentationRabbit polyclonal antigen affinity purified antibody in PBS. Anti-GST has not been adsorbed. Liquid.
      Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Aggrecan Antibody is an antibody against Aggrecan for use in IF, IH(P), IP & WB.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry(Paraffin):
      A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments.

      Immunoprecipitation:
      A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100.
      Biological Information
      ImmunogenGST fusion protein containing amino acids 1177-1326 of mouse aggrecan.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAB1031 is specific for mouse aggrecan; other species have not been tested.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Expected to cross react with Human and rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
      Gene Symbol
      • ACAN
      • MSK16
      • SEDK
      • CSPCP
      • AGCAN
      • AGC1
      • CSPG1
      • CSPGCP
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
      SIZE: 2415 amino acids; 250193 Da
      SUBUNIT: Interacts with FBLN1 (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      TISSUE SPECIFICITY: Restricted to cartilages.
      DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton.
      DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.
      PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
      DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.
      SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain.
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AB1031 04053252623196