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ABC821 Anti-AMBRA1 Antibody

Anti-AMBRA1 Antibody is an antibody against AMBRA1 for use in western blotting, IHC & immunofluorescence.

Anti-AMBRA1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: AMBRA1 UniProt Number: Q9C0C7
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ABC821
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IHC, IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC821
      DescriptionAnti-AMBRA1 Antibody
      Alternate Names
      • Activating molecule in BECN1-regulated autophagy protein 1
      • AMBRA1
      Background InformationAutophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation (3), is in turn regulated by AMBRA1. AMBRA1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of AMBRA1 in cultured cells though RNA interference decreased the level of rapamycin and nutrient starvation-induced autophagy (4). Multiple isoforms of AMBRA1 are known to exist.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-AMBRA1 Antibody is an antibody against AMBRA1 for use in western blotting, IHC & immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: 5 μg/mL from a representative lot detected AMBRA1 in human brain tissue.

      Immunofluorescence Analysis: 20 μg/mL from a representative lot detected AMBRA1 in human brain cells.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the N-terminus of human AMBRA1.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • AMBRA1
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~150 kDa observed. Uncharacterized bands may be observed in some lysate(s). Uniprot describes 6 isoforms produced by alternative splicing ranging between ~84 kDa and ~143 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 2 μg/mL of this antibody detected AMBRA1 in rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC821 04053252943829