Membrane NMWL, kDa	Protein Solute	Protein Retentate Volume, mL	Protein Retentate Recovery, %
3	Cytochrome c, 12.4 kDa (0.25 mg/mL)	0.19	98.7
10	10 Cytochrome c, 12.4 kDa (0.25 mg/mL)	0.20	95
30	30 BSA, 67 kDa (1 mg/mL)	0.20	95
50	50 BSA, 67 kDa (1 mg/mL)	0.08	94
100	100 IgG, 156 kDa (1 mg/mL)	0.34	91
4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min).