Cell cultures, by definition, inhabit the unsteady ground between tight, reproducible experimental control and the ability to simulate the complex and dynamic environments found
in vivo. This webcast reveals how pioneering microfluidic technology enables the precise manipulation of physiologically relevant micro-environments, helping researchers connect cellular mechanisms with phenotypes and disease states.
Microfluidic cell culture chambers allow crucial parameters such as media flow, temperature and gas environment to be automated. This enables researchers to recreate the mass transport environment of tissues, elicit cell responses to dynamic solution changes and enable long-term perfusion culture – a real-time, live cell analysis with unprecedented control.
The first speaker, Dr. Andrew Ball, will review a specific workflow involving microfluidic chambers used with a fluorescent microscope and image analysis software, to provide insights for live cell dynamic applications such as host-pathogen interactions, cancer cell autophagy, and cell migration in a standardized format.
Then, Dr. KC Huang, will discuss how specific microfluidic techniques have allowed him to study damped oscillations of bacterial growth in response to osmotic shock. Dr. Huang will describe the advantages of real-time, live cell analysis in a microfluidic context.
In this webinar you learn:
- The advantages and challenges of using microfludic cell culture tools in cell biology
- Techniques that can be used to develop and quantify in vitro cell models for predictive cell analysis.