Pellicon® 2 Ultrafiltration Cassettes
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Documentation
Referencias bibliográficas
| Visión general referencias |
|---|
| Cleaning and Process Qualification Studies for Plasma Protein Products with Tangential Flow Ultrafiltration Pellicon 2 Cassettes Marty Siwak, Robert Blanck, Aline Seilly, Charles Christy, Ken Dibble, Millipore Corporation Michael Morey, Keith Schokman, CSL Bioplasma; Plasma Protein Biotechnology meeting Plasma Protein Biotechnology meeting 1999 |
| Linear Scale Ultrafiltration van Reis, Robert, Elizabeth M. Goodrich, Christine L. Yson, Leah N. Frautschy, Stephen Dzengeleski, and Herb Lutz Biotechnology & Bioengineering (September 1997): 737-746. 1997 |
| Clean-Water Permeability as a Determinant of Cleaning Efficacy in Tangential-Flow Filtration Systems Michaels, Stephen L.; RP016 RP016 1993 |
| Validation of Tangential Flow Filtration (TFF) Systems Michaels, Stephen L. Journal of Parenteral Science and Technology (Sept/Oct 1991). 1991 |
Preguntas frecuentes
| Pregunta | Respuesta |
|---|---|
| What are turbulence promoters? | Turbulence promoters are screens that are inserted into the feed and/or filtrate channels in TFF modules in order to increase the "turbulence" in the channels of the module and to reduce the occurence of concentration polarization (the gel layer that can form on membranes therefore slowing flux). These turbulence promoted channels allow for higher fluxes with lower pumping requirements which makes them more efficient than open channels without turbulence promoters. |
| What TFF filters can I use to concentrate and recover cells? | We have 0.45um and 0.65um Durapore membranes in Pellicon and Prostak configurations for concentrating large volumes of cell solutions. |
| Are the TFF membranes autoclavable or steam sterilizable? | The membranes for Pellicon® 3 cassettes are not autoclavable or steam sterilizable. However, a chemical sanitization can be done; refer to the user guide for recommendations. |
| What does linear scalability mean? | Pellicon filters scale-up easily and reliably from laboratory to the production plant. By ensuring every flow channel has the same length, height and turbulence promoter as well as flow direction, this maintains the same ultrafilter performance at all scales. This leads to reliable and rapid translation of processes from the lab to the manufacturing scale. Additionally, every Pellicon 2 filter operates with the same pressure drop, flow velocity and concentration profile for true, rapid and simple linear scale-up. |
Productos y aplicaciones relacionados
Familias de productos
Pellicon® XL 50 Ultrafiltration Cassettes - UltrafiltrationTrue Linear scalability with UF membranes with the same flow path and channel height as larger Pellicon® 2 cassettes. The Pellicon® XL 50 allows process development and scaling studies.Más información >> |
Pellicon® 3 CassettesAdvanced, high performance cassettes ideally suited to today's demanding filtration processes that require more rigorous operating pressures, temperatures and caustic cleaning regimesMás información >> |
Pellicon® Cassette Acrylic Holder and AssemblyThese acrylic holders operate a selection of Pellicon® Cassettes for tangential flow microporous filtration and ultrafiltration.Más información >> |
Pellicon® 2 Microfiltration CassettesA new standard in cassette style tangential flow filtration devices.Más información >> |
Pellicon® Cassette Stainless Steel Holder and AssemblyDesigned for sanitary applications. Can be used to expand existing cassette ultrafiltration (CUF) systems or to replace existing holders in CUF systems.Más información >> |
Pellicon® Mini Cassette HolderSimple design makes process development and small scale pharmaceutical manufacturing simple and efficient.Más información >> |
Productos relacionados por: Application Facete
| Antibody Purification |
| Dialysis and Filtration |
Productos relacionados por: Brand Facete
| Pellicon® |
Categorías
| Biopharmaceutical Manufacturing > Downstream Processing > Ultrafiltration > Pellicon Cassettes > Pellicon 2 Cassettes |
Pellicon® 2 cassettes set the standard in cassette-style tangential flow filtration devices. The Pellicon product line was designed with process scale-up and applications development in mind.
Millipore’s Biomax® polyethersulfone and Ultracel® regenerated cellulose membranes have void-free structures that guard against leakage of solutes through microdefects normally associated with voids beneath the thin skins of conventional UF membranes. These void-free membranes are more permeable, resulting in high-flux with equivalent or superior product retention. These void-free membranes provide the advantages of fast, high yield processing and smaller systems.
Benefits
- Leading-edge void- free membranes to match virtually any separation challenge
- Short flow path for higher flux and higher resolution separation capability
- Choice of flow channel configuration providing process optimization capability
- Predictable, fast, scale-up
- True linear scalability from laboratory size modules to industrial assemblies for processing thousands of liters
- Brings higher resolution, improved yields and superior back-pressure resistance
- Ultracel membranes are composite membranes cast on a microporous substrate for defect-free membranes with superior adhesion.
Applications
Concentration, desalting or buffer exchange of:
- Protein solutions
- Polysaccharide solutions
- Virus suspensions
Harvest, washing or clarification of:
- Cell cultures and lysates
- Colloidal suspensions
- Viral cultures
| Screen Selection Guidelines | |||
| Solution Type | Screen Type | ||
| Dilute protein solution or low viscosity solutions (mAbs, interferons) |
A screen (tight screen) |
||
| Concentrated protein solutions or high viscosity solutions (IgG, biopolymers) |
C screen (coarse screen) |
||
| High viscosity solutions (polysaccharides, certain microfiltration or clarification applications) | V screen (loose screen) |
||
| Normalized Recirculation Rates | |||
| Parameter | Typical ∆P | ||
| Screen Type | A | C | V |
| Recirculation Rate | 4/6 L/min/m² | 5/35 L/min/m² | N/A |
| Differential Pressure | 1.4/20 bar/psi | 0.4/6 bar/psi | N/A |
| Membrane Selection Guide | ||
| Membrane Type | Materials | Benefits |
| Biomax® | Modified polyethersulfone | Highest flux ultra-filtration membrane Excellent chemical resistance Void-free structure for higher yield and reliability |
| Ultracel® | Regenerated cellulose | Extremely low protein binding hydrophilic membrane (ideal For Protein Solutions < 20 G/l) Highest product recovery and improved performance with Difficult To Process Streams (antifoams, lipids, protein transmission applications) |
| Membrane Selection Chart | |||||
| Approximate Molecular Weight (range of solutes retained >99%, kD) |
Membrane | NMWL (kD) or Microns | Membrane Material | pH Range | |
| Biomax® Membranes - Void-free for Higher Yield and Reliability | |||||
| 12 – 25 | (growth factors, hormones) | Biomax-5 | 5 | Modified polyethersulfone | 1 – 14 |
| 25 – 50 | (growth factors, hormones) | Biomax-8 | 8 | Modified polyethersulfone | 1 – 14 |
| 50 – 100 | (albumin, hemoglobin) | Biomax-10 | 10 | Modified polyethersulfone | 1 – 14 |
| 100 – 140 | (enzymes) | Biomax-30 | 30 | Modified polyethersulfone | 1 – 14 |
| 140 – 300 | (IgG’s) | Biomax-50 | 50 | Modified polyethersulfone | 1 – 14 |
| 300 – 500 | (small viruses and antigens) | Biomax-100 | 100 | Modified polyethersulfone | 1 – 14 |
| > 500 | (IgM’s, large viruses) | Biomax-30 | 300 | Modified polyethersulfone | 1 – 14 |
| > 0.03 µm | (large viruses, colloids, particulates) | Biomax-500 | 500 | Modified polyethersulfone | 1 – 14 |
| > 0.03 µm | (large viruses, cells, colloids, particulates) | Biomax-1000 | 1000 | Modified polyethersulfone | 1 – 14 |
| Ultracel Membranes – for High Recoveries | |||||
| 8 – 18 | (proinsulin, hematopoietic factors) | PLCCC | 5 | Regenerated cellulose | 2 – 13 |
| 18 – 60 | (hemoglobin, enzymes) | PLCGC | 10 | Regenerated cellulose | 2 – 13 |
| 60 – 200 | (monoclonal IgG’s) | PLCTK | 30 | Regenerated cellulose | 2 – 13 |
| 200 – 500 | (small viruses, viral antigens) | PLCHK | 100 | Regenerated cellulose | 2 – 13 |
| > 500 | (large viruses, IgM’s) | PLCMK | 300 | Regenerated cellulose | 2 – 13 |
| > 0.03 µm | (large viruses, cells, colloids, particulates) | PLCXK | 1000 | Regenerated cellulose | 2 – 13 |
| Specifications | |||
| Temperature Range | |||
| Mini, Cassette and Maxi: | 4 to 50 °C | ||
| Maximum Forward Transmembrane Pressure | |||
| Device Area (m²) | Biomax® Membrane | Ultracel® Membrane | |
| 0.1 | 6.8 bar (100 psi) Max | 6.8 bar (100 psi) Max | |
| 0.5 | 6.8 bar (100 psi) at 30 °C | 3.4 bar (50 psi) at 30 °C | |
| 2.5 | 6.8 bar (100 psi) at 30 °C | 3.4 bar (50 psi) at 30 °C | |
| Maximum Reverse Transmembrane Pressure | |||
| Device Area (m²) | Biomax® Membrane | Ultracel® Membrane | |
| 0.1 | 0.33 bar (5 psi) | 0.33 bar (5 psi) | |
| 0.5 | 0.33 bar (5 psi) | 0.33 bar (5 psi) | |
| 2.5 | 0.33 bar (5 psi) | 0.33 bar (5 psi) | |
| Dimensions | |||
| Device | Width | Length | Thickness |
| Mini | 5.6 cm | 21 cm | 1.5 cm (V screen-2.16 cm) |
| Cassette | 17.8 cm | 21 cm | 1.5 cm (V screen-2.16 cm) |
| Maxi | 17.8 cm | 21 cm | 7.6 cm (V screen-9.0 cm) |