PCR Cleanup Filter Plates
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Descripción
Our PCR filter plates are fast, automatable solutions for high-throughput PCR purification. Available in 96- and 384-well formats and a µ96-well format for small volumes, MultiScreen® PCR plates use a size-exclusion membrane and vacuum filtration to provide a one-step protocol with excellent results. No centrifugation or precipitation is required. Full sample recovery from the top of the plate enhances compatibility with liquid handling systems. MultiScreen® PCRµ96 and MultiScreen®PCR384 filter plates provide high recovery on even the smallest miniaturized reactions. Working volumes <150 µL conserve reagents and save money. For volumes >150 µL, use MultiScreen®PCR96 96-well filter plate.
Features & Benefits
- High purity and high recovery
- No centrifugation or precipitation
- Fast processing times, automation compatible
- MultiScreen® PCRµ96 filter plate recommended for small fragments (1-150 bp)
Protocol
| 1. | Load PCR reactions. |
| 2. | Filter with Millipore vacuum manifold for 5–10 minutes or until wells are dry. |
| 3. | Add water or buffer to each well. Agitate by shaking or pipetting. Retrieve purified samples by aspiration. |
Selection Guidelines
| Specifications | |||||
| Product name | Reaction volume |
Number of samples |
Mode of operation |
Recommended applications |
Features |
| MultiScreen® PCRµ96 Filter Plate | 20 – 150 µL | 96 | Vacuum | Sequencing Genotyping Microarray |
• Highly concentrated final sample • Reaction miniaturization |
| MultiScreen® PCR96 Filter Plate | 150–300 µL | 96 | Vacuum | Microarray | • Volumetric capacity |
| MultiScreen® PCR384 Filter Plate | 20–100 µL | 384 | Vacuum | Sequencing Genotyping Microarray |
• Highly concentrated final sample • Reaction miniaturization • Ultra-high throughput |
Recovery
| Product name | Recovery of 137 bp PCR fragment* | Recovery of 301 bp PCR fragment* | Recovery of 657 bp PCR fragment* | Recovery of 1159 bp PCR fragment* | % Primer removal (20 bases), 100 µL volume |
| MultiScreen® PCRµ96 Filter Plate | + | ++ | ++ | ++ | 99.8% |
| MultiScreen® PCR96 Filter Plate | -- | + | ++ | ++ | 98.7% |
| MultiScreen® PCR384 Filter Plate | + | ++ | ++ | ++ | 99.5% |
| -- Not Recommended | |||||
| + Good Recovery | |||||
| ++ Best Recovery | |||||
| *Results will vary based on starting concentration, load, and buffers used. Data obtained using the following concentrations: 137 bp: 10 ng/µL 301 bp: 30 ng/µL 657 bp: 55 ng/µL 1159 bp: 71 ng/µL |
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Performance
Get high reproducibility with MultiScreen PCR96 Filter Plate
Agarose gel showing reproducibility of recovery of a 500 bp PCR product with MultiScreen PCR96 filter plate.
Long Sequencing Reads
Representative electropherogram from a 301 bp PCR product purified using MultiScreen PCR96 filter plate (Thermo Sequenase® II dye terminator).
Suitable for Microarrays
Enlarged section of a 19,299 element microarray (image reproduced with permission from Dr. John Quakenbush, The Institute for Genomic Research). High quality microarrays can be manufactured using PCR products purified with MultiScreen PCR products. Reference: Hegde, P., R. Qi, K. Abernathy, C. Gay, S. Dhaarp, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee and J. Quakenbush. 2000. A concise guide to cDNA microarray analysis. BioTechniques 29: 548–562.
Applications
PCR Cleanup, Sequencing, Genotyping, Microarray, RNAi Purification, Microarray Purification, Genomic DNA Concentration