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04-1624 Anti-Centrin Antibody, clone 20H5

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04-1624
100 µg  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1624
      DescriptionAnti-Centrin Antibody, clone 20H5
      Alternate Names
      • Caltractin isoform 2
      • EF-hand protein
      • calcium binding protein
      • centrin 1
      • centrin, EF-hand protein, 1
      Background InformationCentrin is a key regulator of centriole duplication. This protein is localized to the centrosome of interphase cells. During mitosis, centrin redistributes to the spindle poles, consistent with dynamic changes of the centrosome during the cell cycle. Current research suggests centrin exerts spatial and temporal control over centriole duplication through its role as a component of centriole precursor structures and through periodic cell-cycle specific changes in its abundance.
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Centrin Antibody, clone 20H5 is a Mouse Monoclonal Antibody for detection of Centrin also known as Caltractin isoform 2, EF-hand protein & has been validated in WB, IP, ICC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: Manandhar, G., et al. (1999). Cell Motil Cytoskeleton. 43(2):137-144.
      Biological Information
      ImmunogenRecombinant protein corresponding to the C-terminus of Chlamydomonas Centrin.
      EpitopeC-terminus
      Clone20H5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the C-terminus of Centrin.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.
      Gene Symbol
      • CEN1
      • CETN
      • Centrin-1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Plays a fundamental role in microtubule-organizing center structure and function.

      SUBUNIT STRUCTURE: Monomer By similarity.

      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton › centrosome. Note: Centrosome of interphase and mitotic cells.

      MISCELLANEOUS: Binds two moles of calcium per mole of protein.

      SEQUENCE SIMLARITIES: Belongs to the centrin family.

      Contains 4 EF-hand domains.
      Molecular Weight ~ 20 kDa
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 1 µg/ml of this antibody detected Centrin on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Número de referencia GTIN
      04-1624 04053252672200