Millipore Sigma Vibrant Logo

105387 Leishman's eosin methylene blue solution modified

View Products on Sigmaaldrich.com
105387
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
1053870500
Retrieving availability...
Limited Availability
Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Plastic bottle 500 ml
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      1053871022
      Retrieving availability...
      Limited Availability
Limited Availability
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic bottle 20 x 1 l
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          Catalogue Number105387
          DescriptionLeishman's eosin methylene blue solution modified
          OverviewThe staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright's eosin methylene blue solution and May-Grünwald's eosin methylene blue solution,this ready-to-use solution is used for staining of blood and bone marrow smears and clinical cytological specimens. The typical resulting color of cell nuclei is red to purple, which depends on the buffer tablets (Buffer tablets pH 7.2 or Buffer tablets pH 6.8). The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances.
          A 500 mL bottle of Leishman's eosin methylene blue solution are sufficient for staining up to 1600 slides in Hellendahl cells.As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          References
          Product Information
          HS Code3212 90 90
          Quality LevelMQ400
          Applications
          Applicationfor microscopy
          Biological Information
          Physicochemical Information
          Boiling point65 °C (1013 hPa)
          Density0.79 g/cm3 (20 °C)
          Explosion limit5.5 - 44 %(V) Methanol
          Flash point12 °C
          Ignition temperature455 °C Methanol
          Vapor pressure128 hPa (20 °C)
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Hazard Pictogram(s) Hazard Pictogram(s)
          Hazard Pictogram(s)
          Hazard Pictogram(s)
          Hazard Statement(s)H225: Highly flammable liquid and vapour.
          H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
          H317: May cause an allergic skin reaction.
          H370: Causes damage to organs.
          Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
          P233: Keep container tightly closed.
          P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
          P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
          P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
          P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
          P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
          Signal WordDanger
          Storage class3 Flammable liquids
          WGKWGK 2 obviously hazardous to water
          Disposal1
          Strongly contaminated halogen-free organic solvents: container A.
          Safety Information
          Categories of dangerhighly flammable, toxic
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Packaging Information
          Transport Information
          Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
          Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
          Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
          Supplemental Information
          Specifications
          Suitability for microscopy (Blood smear)passes test
          Erythrocytesreddish
          Nucleiviolet
          Eosinophilic granulesbrick-red
          Neutrophilic granuleslight violet
          Basophilic granulesdark violet
          Lymphocyte cytoplasmnblue
          Monocyte cytoplasmgrey-blue
          Thrombocytesviolet
          Global Trade Item Number
          Catalogue Number GTIN
          1053870500 04022536063911
          1053871022 04022536707358

          Related Products & Applications

          Alternative Products

          Catalogue Number Description  
          101350 Leishman's eosine methylene blue Show Pricing & Availability

          Accessories (Primary)

          Catalogue Number Description  
          111374 Buffer tablets pH 6.8 Show Pricing & Availability
          106009 Methanol Show Pricing & Availability
          109468 Buffer tablets pH 7.2 Show Pricing & Availability

          Related Products By: Application Facete

          Categories

          IVD/OEM Materials & Reagents > Microscopy > Hematology > Rapid Stainings, Hemacolor®
          IVD/OEM Materials & Reagents > Microscopy > Hematology > Hematological Staining Solutions